342 



Mary Davies Swartz, 

 Examination of Urine. 



ROTATION. 



ESTIMATION OF AMOUNT 

 OF SINISTRIN EXCRETED.* 



January 11,10:30 A.M. 



January 11, 3 P.M 



January 12, 9:30 A.M.. 

 January 13, 9:30 A.M.. 

 January 14, 9:30 A.M.. 



* Calculating for slnlstrln 



Injection 



88 



127 



116 



-0.48° 



-2.04° 

 -0.48° 

 - 0.48° 



Grams. 



2.7 



= —29.1°. 



Experiment B. A dog weighing 4.6 kg. received 108 cc. of sinis- 

 trin solution, containing 2.3 grams pure substance. The rotation of 

 this solution was —1.38° in a 200 mm. tube. No reducing sub- 

 stance was detected in the urine at any time. The changes in rota- 

 tion are shown in the following table: 



Examination oj Urine. 



* Calculating for sinlstrln [a] d = — 29.1° 



In all these experiments, the sinistrin was isolated and identified as 

 a levo-rotatory carbohydrate, yielding reducing sugar on hydrolysis. 

 It was apparently excreted quantitatively in every case. 



FEEDING EXPERIMENTS. 



Methods and Technique. 



Feeding experiments were conducted with dogs and human sub- 

 jects, under conditions as nearly normal as possible. The dogs were 

 kept in metal cages, arranged for the separate collection of urine and 

 faeces. They were fed once a day, on a uniform weight diet, consist- 

 ing of chopped lean meat, lard, and cracker meal, in suitable portions 



