202 LIVERPOOL MARINE BIOLOGY COMMITTEE REPORT. 



tissues, has the disadvantage of destroying any natural 

 colours the animal may have, and it also appears to render 

 the specimens somewhat brittle. Glycerine, besides possess- 

 ing most valuable preservative qualities itself, has the happy 

 advantage of mixing in any proportion with both alcohol and 

 water, and it has been found that a solution composed as 

 follows — Water 1 part, Proof Spirit 2 parts, and Glycerine 

 1 part, with 1 per cent, of Carbolic Acid added, is admirably 

 adapted for preserving these small Crustaceans. Any 

 tendency that the glycerine alone might have to dissolve out 

 carbonate of lime is probably counteracted by the addition of 

 the spirit and water. 



From either the picric acid and alcohol solution (after 

 washing), or from the glycerine mixture, the objects may 

 without further preparation be at once mounted in Farrant's 

 solution as permanent slides, by which their natural charac- 

 teristics are maintained intact. 



The collection of Copepoda includes nineteen species, of 

 which thirteen are previously unrecorded as belonging to this 

 locality. Three, at least, are altogether new to Britain, and 

 one is possibly an addition to science. 



The tow-netting observations made at Port Erin, at 

 various times of the day and evening, do not seem to show 

 any marked variation according to the time ; the Copepoda 

 being very much the same in gatherings taken in the middle 

 of the day and after sundown. 



My thanks are due to Dr. G. S. Brady, F.R.S., for 

 having kindly examined and identified some of the more 

 difficult specimens, and for additional information in regard 

 to some of the species described in his Ray Society Mono- 

 graph and " Challenger" Report.* 



* A Monograph on the British Copepoda, Eay Society, 1878 and 1880. 

 Keport upon the Copepoda collected during the Voyage of H.M.S. * Chal- 

 lenger,' ZooL CJiall. Exp., Part xxiii, 1883. 



