434 E - WACE CARLIER 



animais used in determining the most reliable fixing agent, and they were 

 many. Nor it is peculiar to the rat, as it occurs in the livers of many of the 

 smaller domestic animais, and apparently also in that of man. 



Many cells also contain numerous fine, highly refractile particles of a 

 greenish grey colour, situated mostly between the nucleus and the bile 

 capillary, but occasionally scattered throughout the cytoplasm. They hâve 

 not been observed within the nucleus. Thèse particles vary considerably in 

 size, but are always minute; they appear similar to those recorded by 

 me (') in the liver of the hedgehog during hibernation, and well known to 

 exist in this gland in the amphibia and hibernating reptiles during their 

 periods of torpor. 



The cytoplasm contains no fat. 



The nucleus, of which there may be two, is rounded or oval in shape, 

 plump, rather poor in chromatin when compared with the nuclei of some 

 gland cells; it stains deeply and is distributed partly under the capsule in 

 small irregular karyosomes and partly upon the nucleoli in the form of 

 crescentic masses. 



The linin network is very fine and difficult to recognise. 



The nucleoli, which are often multiple, are of moderate size, do not 

 stain bright red with eosine, and occupy various positions in the nucleus, 

 not unfrequently they lie close to the nuclear envelope and may occasionally 

 be seen causing it to bulge somewhat as if in process of passing through. 

 The extrusion of nucleoli is, however, not at ail a conspicuous feature of 

 cells of the fasting animal, and it is not at any time accompanied by expul- 

 sion of the chromatin that may hâve been adhèrent to them whilst centrally 

 placed. 



The liver cells immediately bordering on the portai tracts are much 

 smaller than those in the remainder of the lobule, being only about half as 

 big, fig. 2 et 3; they are usually uni-nucleated and characterised by a 

 denser and doser arrangement of the cytoplasm, which causes them to stain 

 somewhat more deeply than the others; they contain some structureless 

 material, but no refractile granules as far as my observations go. 



The capillaries between the liver cells are not dilated and the stellate 

 cells, which are an inconspicuous feature in the liver of thèse animais, 

 offer no peculiarities for comment. 



(') Carlier : Contributions to the histology of the hedgehog; Journ. Anat. and Physiol , v. 27. 



