CONCERNING THE SECRETION OF FERMENTS BY THE LIVER CELLS 435 



Appearances présentée by the liver cells i hour afterfeeding. 



The cell walls are distinct, the cells well filled without much vacuola- 

 tion and the structureless material, which does not stain so vividly as in 

 the fasting animal, is abundant, though arranged in smaller masses. The 

 highly refractile granules hâve entirely disappeared, and, as they do not 

 again make their appearance throughout the whole séries of experiments, 

 will not again be alluded to. 



The nuclei are swollen, pale looking and either contain very little 

 chromatin, or this has been so altered that it no longer stains vividly with 

 methyl blue but exhibits a pale slate colour instead. The nucleoli, therefore, 

 appear more prominent, though they hâve scarcely increased in size and 

 are almost invariably situated just under the nuclear membrane, or are in 

 process of passing through it, or even lying in the cytoplasm beyond it. 

 The présence of an iron and phosphorous holding substance situated in the 

 cytoplasm, in the immédiate vicinity of, and derived from, the nucleus is 

 easily revealed by Me Allums method. The capillaries are not enlarged 

 and the stellate cells exhibit no change. 



Appearances presented by the liver cells 2 hoitrs afterfeeding. 



The cell outlines are distinct, though pale and somewhat irregular, 

 the cytoplasm is mostly aggregated round the margins and about the 

 nucleus, leaving the greater part of the cell body clear, i. e. there is much 

 vacuolation, which in some cells is carried to an extraordinary degree; the 

 structureless material is abundant, vividly coloured, and situated, not in 

 the vacuoles, but in the cytoplasm, which also stains more deeply with 

 eosine than previously, having a reddish rather than a greyish tint. 



The nuclei are shrunken, pale and almost devoid of chromatin, though 

 a few in the outer zone of the lobules are swollen and becoming clouded ; 

 the nucleoli are few in number and much prezymogen can be revealed 

 around the nuclei by appropriate methods. The capillaries are dilated. 



Appearances presented by the liver cells 3 hoitrs afterfeeding. 



The cells are no longer shrunken but swollen to some extent with 

 distinct outlines, and contain fewer and smaller vacuoles, but the structure- 

 less material is less abundant, more diffuse, and less vividly stained than 

 in the preceeding préparations. 



