CONCERNING THE SECRETION OF FERMENTS BY THE LIVER CELLS 439 



round deeply staining particles adhèrent to the linin network; this is an 

 early stage of fatigue. 



Others are small with somewhat wrinkled outlines and little chromatin. 

 The nucleoli are large, often quite close to the nuclear envclope, and in 

 one or two cases migration of them into the cytoplasm was observed. The 

 nuclei of the small cells round the portai tracts are ail wrinkled. The 

 capillaries are dilated. 



Appearances presented by the liver cells 2 hours after feedbig, fig. 4. 



The outlines of the cells are somewhat more distinct and irregular in 

 places, the cytoplasm, which appears composed of small spherules, still 

 stains of a perl grey colour, but exhibits a slightly pinker hue than in the 

 previous spécimens; some small vacuoles are présent hère and there but 

 are nowhere conspicuous objets. 



The small cells round the portai tracts hâve dense cytoplasm of a 

 redder tint than that of the other cells, their nuclei are small, rounded and 

 very clouded, showing that they are recovering after exertion; a pheno- 

 menon exhibited, likewise, by their cytoplasm. In the lobular cells, the 

 nuclei are large, swollen and clouded in the outer zone, whilst in the middle 

 zone they contain pale staining chromatin in small quantity, and in the 

 inner zone they are as in the preceeding spécimens. In this way the lobules 

 are distinctly marked off into three zones, the outer in full repair, the 

 middle with swollen nuclei and slightly vacuolated cytoplasm and the inner 

 with cytoplasm more vacuolated and the nuclei distorted. The structureless 

 material is very scanty, but more abundant in the outer zone cells. The 

 capillaries are dilated. 



Appearances presented by the liver cells 3 hours after feeding. 



The cell outlines are fairly distinct and not distorted, the cytoplasm is 

 arranged in coarse spherules in ail the cells, most closely packed in the 

 small cells lining the portai tracts which stain deeply, less closely packed 

 elsewhere; there are no vacuoles présent except in the immédiate neigh- 

 bourhood of the intra-lobular veins. Ail the nuclei are plump and contain 

 deeply staining karyosomes in normal amount; they appear to be resting. 

 The structureless material is présent in fair quantity throughout the 

 lobules, except in the immédiate neighbourhood of the portai tracts, where 

 it is found in small amount only. The capillaries are not dilated. 



