44a E. WACE CARLIER 



is when the cells require to take up nutritive material from the blood to 

 transform the prezymogen into zymogen and for the reconstruction of 

 nuclear chromatin, at other times they are of normal resting calibre or even 

 reduced in diameter when the cells hâve become swollen with the nutritive 

 material they hâve absorbed. 



Third séries of experiments with a purely carbohydrate diet. 



The diet consisted of starch paste sweetened with cane sugar. The 

 animais were fed upon this mixture for several days previous to the com- 

 mencement of the experiments, but never at any time exhibited enthusiasm 

 for their food and only ate sparingly of it. The methods adopted for the 

 préparation of the spécimens were identical to those employed in the 

 previous experiments. 



Appcarances présentai by the liver cells i hour after feeding, fig. 7. 



The cell outlines are distinct and the cells well filled with a pink 

 staining cytoplasm precipitated in the form of large spherules (largest in 

 the inner zone cells) many of which are of ovoid shape; they stain peculiarly 

 with toluidin blue-eosine mixture, presenting a solid looking dull yellowish 

 body to the margins of which a purplish staining material is adhèrent in 

 irregular patches. The cells présent some vacuolation, but the vacuoles 

 are small and scattered, and the structureless material, which is abundant 

 only in some cells, is diffusely distributed. The nuclei throughout the 

 préparations are swollen and clouded, though those of the small cells round 

 the portai tracts are less so than the others, and the cytoplasm of thèse 

 cells is much pinker than that of the larger ones. The nucleoli are mostly of 

 small size and show little tendency to migrate; the capillaries are dilated. 



Evidently the first sécrétion of prezymogen is over and has lasted less 

 than an hour, no doubt because the animais demonstrated no keen désire 

 for their food and took little interest in it, though they had been fasting 

 for the previous twenty four hours. 



Appcarances presented by the liver cells 2 hours after feeding. 



The cell outlines are less distinct than in the previous spécimens, and 

 the cytoplasm fills the cells almost without vacuolation, it stains of a 



