CONCERNING THE SECRETION OF FERMENTS BY THE LIVER CELLS 447 



Appearances présentée by the Hver cells 4 hours afterfeeding. 



The cell outlines are very distinct, the cytoplasm is not vacuolated 

 but contains much structureless material, espetially near the nuclei where 

 it is clustered into masses ; the spherules are numerous though small 

 and stained dull violet, giving a violet hue to the whole cell, which con- 

 tains very little precipitated albumin. The nuclei are quite at rest and 

 rounded with small nucleoli, the capillaries are small. 



The small cells round the portai tracts are very dense with a ground 

 glass-like appearance, they contain many small spherules with dark 

 outlines. 



Appearances présentai by the Hver cells Shows afterfeeding. 



The cell outlines are distinct and of a pale grey colour, the cytoplasm 

 is not vacuolated though open in appearance and built up of small spherules 

 that stain homogeneously of a dull purple colour; the structureless material 

 is abundant but diffusely scattered giving a clouded appearance to the 

 cytoplasm. 



Under a low magnification the Hver appears parti-coloured, being red 

 round the portai tracts and pale lilac elsewhere; in the outer zone, which 

 is so red, the nuclei are somewhat irregular in shape with purplish chroma- 

 tin under their envelopes and big pale-centred nucleoli, two or more in one 

 nucleus. The transition between the outer and middle zones is somewhat 

 abrupt. In the middle zone the nuclei are swollen, somewhat clouded, with 

 incipient karyosomes; they hâve undoubtedly almost recovered after sécré- 

 tion, those of the inner zone are swollen and clouded, i. e. they show an 

 early stage of repair, their nucleoli are small and the cells in which they 

 lie vacuolated. The capillaries are considerably dilated. 



Thèse préparations are most remarkable for the extraordinary number 

 of large nucleoli présent in the outer zone cells, many of which are migrating, 

 and for the fact that somewhere between the 4 th and 5 th hours there must 

 hâve been nuclear activity, recovery from which is already well on the way. 



Appearances presented by the Hver cells 6 hours after feeding, fig. 12. 



The cell outlines are fairly distinct, the cells surrounding some of the 

 smaller portai tracts are stained very red, elsewhere they présent a uniform 

 greyish colouration. A few contain large vacuoles, the others présent none, 



oo 



