448 E. WACE CARLIER 



but being made up of larger dull violet-coloured spherules with darker 

 edges hâve a dense appearance ; the structureless material which is arranged 

 either diffusely or in small masses is somewhat less abundant than at the 

 end of the fifth hour. Most of the nuclei are still somewhat swollen, with 

 newly formed karyosomes and large migrat'ing nucleoli and those of the 

 inner zone are a little clouded, having not yet quite recovered from their 

 transient exertions. The capillaries are of moderate size. 



The great number of large, pale centered nucleoli in progress of migra- 

 tion into the cytoplasm is again the chief feature exhibited by the cells at 

 this hour, thèse nucleoli are if anything more numerous and striking than 

 at the fifth hour. 



Appearances presented by the liver cells 7 hours after feeding. 



The cell outlines are somewhat indistinct, the reddish violet cytoplasm 

 is close in texture, consists of médium sized spherules of a dull violet 

 colour with darker edges, closely packed in the outer zone but less crowded 

 elsewhere, and contains structureless material in abundance, sometimes 

 arranged in masses but more often diffusely scattered. The cells are now 

 resting as are also their nuclei, except in the inner zone where they are 

 still somewhat swollen, though clear and contain newly formed karyo- 

 somes ; the capillaries are narrow. 



Appearances presented by the liver cells 8 hours after feeding. 



In this animal digestion from some cause or another seems to hâve 

 proceeded somewhat more slowly than in the others, the appearances 

 presented tallying with those of the sixth rather than with those of the 

 seventh hour, but in another hour's time the liver would undoubtedly hâve 

 come to rest. 



This séries of experiments again shows clearly that the liver is subject, 

 during digestion, to two distinct periods of ferment production ; the first 

 not very intense and soon over, the second not more intense than the first 

 and comparatively quickly recovered from. Probably, therefore, a diet of 

 fat does not call for much assistance from the liver ferments for its 

 digestion. 



The method in which the cytoplasm is precipitated by the fixative is 

 again characteristic, as is, also, the peculiar dull violet coloration which is 



