CONCERNING THE SECRETION OF FERMENTS BY THE LIVER CELLS 449 



given to the spherules with methyl-blue eosine mixture. Another feature 

 worthy of note is the présence of albumin precipitate within the cell 

 vacuoles during the early stages; this has not been noticed in any of the 

 other séries examined. 



Fifth séries of experiraents. 



In this séries the rats after fasting for 24 hours were allowed to smell 

 pièces of raw méat placed outside their cages, but beyond their reach; they 

 manifested a very lively interest in the proceedings and endeavoured in 

 every way to reach the food ; after five minutes the food was removed and 

 one rat prepared, as above detailed, every quarter of an hour. 



Any changes presented by the cells must in this case be due to psychic 

 influence alone. 



Appearances presented by the liver cells 1J4 hour after smclling food, 



FIG. 13. 



The cell outlines are fairly distinct, the violet stained cytoplasm has 

 an open appearance, consists of médium sized spherules of a faintly purple 

 colour and contains much structureless material in large or small masses. 

 The nuclei are rounded, ovoid, or indented, contain abundance of chroma- 

 tin and small nucleoli; hère and there the chromatin of some nuclei is 

 beginning to stain less vividly. The capillaries are somewhat distended. 



Appearances presented by the liver cells 1I2 hour after smellingfood. 



The cell outlines are not very distinct, the cytoplasm appears less 

 dense, stains less intensely and consists of small spherules that do not stain 

 distinctly but give it a » foam like « or blistered appearance that is very 

 peculiar; there is little structureless material présent in the cells and it 

 stains faintly. 



The nuclei are swollen and pale, in the outer zone many are irregular 

 in outline and the nucleoli are larger than before. 



The small cells round the portai tracts are not more deeply stained 

 than the others but contain somewhat larger spherules closely packed 

 together, and their nuclei are swollen and slightly clouded. The capillaries 

 are rather narrow. 



