1914-] CRILE— THE KINETIC SYSTEM. 275 



more later. If the second test was negative, then the positive result 

 of the first test was accepted as conclusive. 



We have recorded sixty-six clear-cut experiments on dogs, which 

 show that after fear and rage, after anaphylaxis, after injections of 

 indol and skatol, of leucin and tyrosin, of the toxins of diphtheria 

 and colon bacilli, of streptococci, and staphylococci, of foreign pro- 

 teins and of strychnin, the Cannon test for adrenalin was positive. 

 The test was negative after trauma under anesthesia, and after intra- 

 venous injections of thyroid extract, of thyroglobin and of the juices 

 of various organs injected into the same animal from which the 

 organs were taken. Placental extract gave a positive test. The test 

 was sometimes positive after electric stimulation of the splanchnic 

 nerves. On the other hand, if the nerve supply to the suprarenals 

 had beenpreviously divided, or if the suprarenals had been previously 

 excised, then the Cannon test was negative, after the administration 

 of each of the foregoing adequate stimuli. Blood taken directly 

 from the suprarenal vein gave a positive result, but under deep mor- 

 phinization the blood from the suprarenal vein was negative, and 

 under deep morphinization the foregoing adequate stimuli were 

 negative. 



In brief, the agencies that in our brain-cell studies were found to 

 cause hyperchromatism followed by chromatolysis, gave positive 

 results in the Cannon test for adrenalin. The one agent which was 

 found to protect the brain against changes in the Nissl substance — 

 morphin — gave a negative result in the Cannon test for adrenalin. 

 After excision of the suprarenals, or after division of their nerve 

 supply, all Cannon tests for adrenalin were negative. 



Histologic Studies of the Suprarenal Glands. 



Histologic studies of the suprarenal glands after the application 

 of the adequate stimuli which gave positive results to the Cannon 

 tests for adrenalin are now in progress and thus far the histologic 

 studies corroborate the functional tests. 



In hibernating woodchucks, the cells of the adrenal cortex were 

 found to be vacuolated, and shrunken. In loo hours of insomnia, 

 in surgical shock, in strong fear, in exhaustion from fighting, in pep- 

 tone injections, in acute infections, the suprarenal glands undergo 



