in Puerto Rico. Voucher specimen: R. E. Schultes 26363, 
December 13, 1972; and 
seeds **March 1975°’— for mature seeds collected from 
the same colony of trees in Puerto Rico. Voucher speci- 
men: R. E. Schultes, S. von R. Altschul et B. Holmstedt 
s.n., December 12, 1974. 
REFERENCE SUBSTANCES: All reference substances have been 
previously described (9). 
ABBREVIATIONS: 
DMT N,N-dimethyltryptamine 
5-MeO-DMT 5-methoxy-N,N-dimethyltryptamine 
5-MeO-MMT 5-methoxy-N,N-monomethyltriptamine 
5-OH-DMT 5-hydroxy-N,N-dimethyltryptamine or bufotenine 
MTHC 2-methyl-1 ,2 ,3,4-tetrahydro-S-carboline 
6-MeO-THC 2-methyl-6-methoxy-1 ,2,3,4-tetrahy dro-6-carboline 
6-MeO-DMTHC 1 ,2-dimethyl-6-methoxy-1 ,2,3,4-tetrahydro-f- 
-carboline 
ISOLATION OF THE ALKALOIpDS: The vegetal material was 
ground and extracted according to a procedure first used by 
Fish et al. (10). 
Gas CHROMATOGRAPHY (GC): The gas chromatographic 
analyses were performed with a Varian Model 2100 GC equip- 
ped with a flame ionization detector system. A 180 x 0.2 cm 
(i.d.) glass column was silanized and packed with 3% OV-I7 
coated on Gas Chrom Q, 100-120 mesh (Applied Science 
Laboratories, State Coll., Pa.). The separations were obtained 
at a column temperature of 190°C with a nitrogen carrier gas 
flow rate of 30 ml per min. The vaporizer and the detector 
temperatures were 250°C and 300°C, respectively. The 
amounts of alkaloids were determined by peak heights using 
5-hydroxy-N, N-dimethyltryptamine as a standard. 
GAS CHROMATOGRAPHY - MASS SPECTROMETRY (GC-MS): An 
LKB Model 9000 GC-MS (LKB-Produkter AB, Bromma, 
Sweden) was used to confirm the structure of the alkaloids. 
Separation was obtained ona 160 x 0.2 cm (i.d.) silanized glass 
column, packed with the same packing material as for the GC 
277 
