analyses but maintained at 170°C. The flow rate of the helium 
carrier gas was 40 ml per min. The ionizing potential and the 
trap current were 70 eV and 60 wA, respectively. The ion 
source was kept at 250°C. 
Table 1. Gas chromatographic and mass spectrometric data for reference 
compounds. 
Compound® R, Mass spectrum® 
DMT oa 58 (base peak), 103, 105, 
130, 143, 188 (M*) 
MTHC 4.3 78, 102, 115, 143 (base peak), 
186 (M*) 
5-MeO-DMT 5.8 58 (base peak), 103, 117. 
160, 173, 218 (M*) 
5-OH-DMT 8.4 58 (base peak), 103, 117, 
146, 159, 204 (Mt) 
4Ror abbreviations, see material and methods. 
bLKB 9000 GC-MS with helium as carrier gas on 3% OV-17 on Gas Chrom 
Qat 170 C. Ry= retention time in minutes. 
“Tonizing potential was 70 eV. m/e values of the major peaks are given. 
MASS FRAGMENTOGRAPHY (MF): In order to confirm the pres- 
ence or absence of minor alkaloids in the plant materials, the 
specific and sensitive method of mass fragmentography was 
used (11). The principles of the technique have already been 
described (12). The mass spectrometer was controlled by a 
PDP-12 computer system. The channels used were focussed 
carefully on the molecular ion of each compound of interest: 
m/e = 204 for 5S-OH-DMT; m/e = 186 for MTHC; m/e = 188 for 
DMT; and m/e = 218 for S-MeO-DMT. During another experi- 
ment m/e = 58 was chosen with two different sensitivities on 
two channels. 
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