« INFECTIVES GRANULES » OF PROTOZOA 



;)39 



are seen in the life histories of Ihe Mastigamœbae so fully de- 

 scribed by Goldschmidt (see also pp. 263-267 and fig. 412 of my 

 book). 



I seb^ct for special notice the reproduction of the amoeba 

 described in detail by Liston ani Martin, not only because it 

 offers a v^ry typical example of Ihe process of internal bud- 

 formalion, but because the observations were made in my labo- 



b 



•> 



^y 



C 



.-N , 



E 



N 



Fig. 1. — Formation of endogenous cJiromidial huds in an amœbn. 



A, specimen with one large and one small bud; B, smaller specimen with 

 one bud; C, a small specimen with one full-sized bud and two small ones; 



D, a specimen with a full-sized bud in process of liberation from the parent; 



E, a bud recently liberated, with its nucleus not fully formed; F, a small 

 amoeba containing a large bud. All the amœbœ contain numerous chro- 

 midia and ingested bacteria scattered in their cytoplasm; N, pi-incipal nu- 

 cleus, which takes no share in the bud-formation; fj, buds in various stages 

 of formation. After Liston and Martin. 



ratory and I am able to vouch for Iheiraccuracy. In the living- 

 condition the amoeba was seen sometimes to divide as a whole 

 into two by binary fission, after apparent disappearance of the 

 nucleus. More frequently the amœba, while moving about and 

 feeding actively, was seen to extrude liny buds from its cyto- 

 plasm, and these buds moved off as small amcebulai which 

 grew to the size of their parent and produced other buds in iheir 

 turn. By both these methods of reproduction, but especially by 



