INFECTIVES GRANULES » OF PROTOZOA 



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rent. The bud continues to grow in size and at such a stage 

 as Ihat figured in fig. 2, H, it only differs from Ihe newly- 

 exlriided amœba-bud (fig. 1, E) in possessing a kiiietonucleus 

 in addition to the principal nucleus. In later stfiges a flagellum 

 grows out fiom Ihe vicinity of Ihe kinetonucleus and the small 

 flagellate may divide, hut grows finally into a trypanosome. 



Fig. 2. — Formation of endogenous buds (« infective Qi anules «), 

 in Trypanosoma rhodesiense. 



A, a Irypanosome containing chromidial grains, one of which (chr.) is 

 seen coming off from the nucleus; B, a trypanosome containing chromidial 

 grains, two of which are coming off from the surface of the body as a bud (6) ; 

 C and D, trypanosomes with chromidial grains and buds (b) in process of 

 liberation, or liberated, from the body; EL, development of the buds after 

 liberation from the parent ; E-H, growth of the bud with formation of the 

 kinetonucleus; I shows the flagellum formed; J, division of small crithidial 

 forms ; K and L, small trypanosomes ; K, still almost crithidial in structure. 

 After Fry and Ranken. 



From the comparison of the budding in the two cases, it is 

 seen that Ihe bud produced by the trypanosome dilTers only 

 from that produced by Ihe amœba : 1** in being very minute 

 anil containing very few chromidia, perhaps but a single grain 

 (which, however, is of larger size in most cases than the grains 



