542 ANNALES DE LMNSUTUT PASTEUH 



in Ihe cytoplasm, and may be produced by a clumping and 

 fusion of smaller chromidial grannies) ; 2° in the apparent 

 absence of cytoplasm. The second of these two features is by 

 no means uncommon in particular stages ot some Prolo/oa, 

 especially Avhen stained by the Romanowsky stain, which, as 

 I have pointed out elsewhere (1909), lends to make the 

 chromatin-elemcnts appear laiger than thcv really arc since 

 the red-'^taining substance oT the combination deposits round 

 th ' parts witch it selects as well as in them. In the micro- 

 gîimeles oï Coccidhnn, for example, the cytoplasmic substance 

 appears to be represented only by the flagella, and in the 

 microgametes of the malarial parasites, when stained by the 

 Homaiiowsky method, no cytoplasm can be discerned. I feel 

 ju-tified, thereloe, in asserting that the bud formation in 

 trypanosonies, as described by Fry and Ranken, dilTers only in 

 dejree, and not in kind, from that already well-known to take 

 place in many amœbap, and other Protozoa; as compared with 

 the process as described by Liston and Martin, the buds of ihe 

 trypauosomes give the impression of being set free in an 

 earlier and more incomplete stage of differentiation than those 

 of amœbce. 



It is not necessary for me to deal in detail with Ihe <( gra- 

 nule-shedding » described by Henry in Hœmogr/garina simondi, 

 since this author has fully recognized the chromidial nature of 

 these formations, and the opinion I have expressed above with 

 regard to trypauosomes applies also, mutatis muiamlis, to the 

 ra-e described by Henry. I may content myself by referring 

 briefly to the suggestions put forward by Henry to the effect 

 th:it the « granules » are to be interpreted as recapitulnting 

 an older phylogenetic condition in the evolution of the Pro- 

 tista. 



1 have always been of the opinion that tlie condition in 

 whicii the chromatin-elements of the Protisl body are in the 

 form of scattered chromiilial grannies was antecedent phylo- 

 genelically to the condition in which the chromatin-grains are 

 concentrated and organized into a delinite nucleus. 1 imagine 

 to myself the more remote ancestor of the Protozaa as a form 

 v'itli well-developed cytoplasm containing scaitered chromidial 

 grains, and that from such a form the definitely cellular cha- 



