The two subspecies of Brunfelsia grandiflora differ 
primarily in the size and form of the flowers and in the 
size of the fruits. Subspecies Schultesi tends to be smaller 
also in habit and leaf size, but there is considerable over- 
lap between the subspecies in these characters. 
The following key will serve to separate the two con- 
cepts: 
A. Corolla tube usually curved toward the apex, 30-45 mm. long, 
2—-3.5 mm. in diameter, opening at mouth linear-oblong; limb 
35-42 mm. in diameter; capsule 17-22 mm. long 
subsp. grandiflora 
AA. Corolla tube usually straight, 18-30 mm. long, 1-2 mm. in 
diameter, opening at mouth elliptic-obovate; limb 20-40 mm. 
in diameter; capsule 10-16 mm. long. . . . subsp. Schultesii 
These two subspecies are both ecologically and geo- 
graphically isolated. The typical subspecies is usually 
found between 900 and 2000 m. elevation in the inter- 
Andean valleys of the Rios Maranon, Huallaga and 
Ucayali; subspecies Schultesit normally occurs at much 
lower elevations between 100 and 900 m. throughout 
the upper Amazon and its tributaries, although isolated 
populations in Bolivia may reach 1800 m. From morpho- 
logical and geographical evidence, it appears that subsp. 
grandiflora has arisen from its much more widespread 
and variable counterpart through isolation in the inter- 
montane valleys. 
Meiotic chromosome numbers have been obtained for 
the subspecies of Brunfelsia grandiflora. hese indicate 
that 2 = 11 for both subspecies, a number in keeping 
with other known counts in the genus. 
n= 11 Brunfelsta grandiflora subsp. grandiflora, T. Plowman 2960, 
~~ USA, Florida, Miami, Cultivated at Fantastic Gardens, 
25 Nov. 1970 (ECON, INPA). 
11 Brunfelsia grandiflora subsp. Schultesiit, T. Plowman et al. 
2404, Colombia, Comisaria de Amazonas, Rio Amazonas, 
near mouth of Rio Loretoyacu and Puerto Narifio. 5 
Feb. 1969 (GH). 
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