126 Chittenden and Gtmxmins — Nature and Chemical 



II. 0-4895 gram substance gave 67-8'='= N at 15-4* C. and 761-6'"'" 

 pressure = 16-50 per cent. N. 



III. 0-4939 gram substance gave 0-0116 gram ash = 2-35 per cent. 



Percentage composition of ash-free substance, 

 C 52-39, H 6-97, N 16-74. 



The amount of myosin obtained from the fish muscle was not at 

 all commensurate with the amount of flesh experimented with. 

 Danilewsky,* however, found in the muscle tissue of the perch 8-56 

 per cent, of myosin as contrasted with 7-5 per cent, in ox muscle. 

 Whether our inability to separate a corresponding amount from the 

 tissue of the cod and halibut was due to the character of the tissue, 

 to its possible staleness, or to inferior methods of separation we 

 cannot say. Certainly, the yield of myosin in oar case was not as 

 great as from the muscle tissue of the ox, calf, lamb or sheep. 



In composition, and in such reactions as we were able to try, the 

 fish myosin was not unlike the myosin from other preparations. 



Myosin F^ from ox muscle. 



A solution in 15 per cent, ammonium chloride was prepared as in 

 preceding cases and the myosin precipitated by addition of a large 

 amount of water. 



One half of the precipitated myosin was washed with water until 

 all ammonium chloride was removed, then with alcohol and ether, 

 and dried for analysis (F'). 



The otlier half of the myosin was washed somewhat with water, 

 not enough to render it insoluble, then dissolved in 10 per cent, 

 sodium chloride solution, reprecipitated by dilution with water, thor- 

 oughly washed and dried (F"). 



The composition of the two products is shown in the accompanying 

 tables. The i-esults show close agreement with each other, and with 

 the preceding preparations. 



Myosin G^ from ox muscle. 



According to Danilejvsky, a 15 per cent, solution of ammonium 

 chloride is stronger than necessary for the extraction of ^myosin from 



* Ueber die Abhanghigkeit der coDtractionsart der muskeln von der mengen ver- 

 haltnissen einiger ihrer Bestandtheile. Zeitschrift fiir physiologische Cliemie. Band 

 vii, p. 124. 



