134 Chittenden and Gurmnins — Nature and Gheudcal 



The two products are seen to be pi'actically identical in compo- 

 sition, and show close agreement with the other preparations of 

 myosin. 



Myosin I, from mitscle of ox. 



This, the last product analyzed, was obtained from an ammonium 

 chloride extract of the washed muscle tissue from a freshly killed 

 ox, by dialysis. After being washed and dried at 110° C. it was ana- 

 lyzed with the results shown in the accompanying table. 



On comparing now the composition of the thirteen preparations of 

 myosin analyzed, there is seen to be a very close agreement through- 

 out. Further, on comparing the average of the analytical results 

 with Hammarsten's figures for fibrinogen, the composition of the 

 two bodies is seen to be almost identical. Compared with egg 

 albumin, the most striking difference in composition is the low 

 content of sulphur, and even if we assume with Danilewsky* that 

 the sulphur of the ash comes from the sulphur of the myosin the 

 correction therefor would not materially lessen the difference. 

 Myosin also contains nearly one per cent, more nitrogen than egg 

 albumin. 



Goagxdation of niyosi^i solutions. 



According to Kuhne,f the coagulation of myosin in a 10 per cent, 

 sodium chloride solution occurs at 55-60° C. WeylJ obtained similar 

 results. Danilewsky§ states that a 12-15 per cent, ammonium chloride 

 solution of myosin becomes somewhat turbid at 42-43° C, decidedly 

 turbid at 45-50° C, while at 55° C. a flocculent precipitate sep- 

 arates. The same investigator also found that the more concentrated 

 the ammonium chloride solution was, the lower the temperature at 

 which the turbidity and separation of a precipitate occurred, although 

 never below 40° C. In common witli other proteid bodies a slight 

 degree of acidity lowers the temperature of coagulation, while a cor- 

 responding degree of alkalinity raises it. According to the recent 



* Zeitschrift fiir Physiologische Chemie, Band v, p. 161. 

 f Lehrbuch der Physiologischen Chemie, p. 275. 

 \ Zeitschrift fiir Physiologische Chemie, Band i, p. 77. 

 § Zeitschrift fiir Physiologische Chemie, Band v, p. 160. 



