Composition of the Myosin of Mtiscle Tissue. 121 



then washed with water, alcohol, and ether, and dried at 110° C. 

 (D"). 



The composition of both products is shown in the accompanying 

 tables. 



From the analytical data it is seen that the two preparations show 

 close agreement, although there are minor differences ; D", for exam, 

 pie, containing a slightly higher percentage of cai-bon and a corres- 

 pondingly lower percentage of nitrogen than D\ Further, D" con- 

 tains less than half as much ash as the other pi-eparation. Both show 

 fairly close agreement with the my(tsins from ox muscle, with per- 

 haps a slightly higher average percentage of nitrogen. 



Myosin E, from fish. 



So far as we are aware, little attention has been paid to the my- 

 osin from fish flesh. Myosin is assumed to be present and is supposed 

 to be of the same general nature as the myosin from other forms of 

 muscle tissue. 



We first ti'ied the separation of myosin from fresh cod (Gadiis 

 callarias or G. morrhna), using 2 kilos, of the fresh, lean tissue ob- 

 tained in market. The final ammonium chloride extract (15 per 

 cent.) failed to give anything more than an insignificant precipitate, 

 either by dilution with water or by saturation of the fluid with 

 sodium chloride. 



A second preparation was attempted from the flesh of the halibut 

 [Hippoglossus vulgaris) ; 2*5 kilos, of fresh tissue, free from fasciae, fat 

 and integument were thoroughly extracted with thymolized water 

 for several days, the chopped tissue being well rubbed up with the 

 water to insure complete removal of soluble albumins, etc. The 

 thoroughly washed fibre was then triturated with a 15 per cent, 

 ammonium chloride solution and allowed to stand in contact with it 

 for 24 hours. The filtered fluid gave a decided precipitate on boiling, 

 and also on addition of salt to saturation. 



Myosin was separated from the ammonium chloride solution by 

 dialysis as a more or less gelatinous precipitate, and was prepared 

 for analysis by washing with thymolized water, and treatment with 

 alcohol and ether. 



Dried at 110° C. until of constant weight, it yielded the following 

 results : 



I. 0-3335 gram substance gave 0*2043 gram H„0 = 6 08 per cent. 

 H and 0-6267 gram C0,= 51-16 per cent. C. 



Trans. Conn. a.cad., Vol. VIII. lu Dec, 1888. 



