118 Chittenden and Oronmins — N'ature and Chemical 



Myosin B^froin ox muscle. 



A solution of myosin in 15 per cent, ammonium chloride was pre- 

 pared from 4 kilos, of fi-eshly killed beef, as described under A. 

 The myosin was then separated from the solution by dialysis, con- 

 tinued until nearly all of the chloride was removed. The last traces 

 of the ammonium salt were separated by filtration of the gelatinous 

 myosin through chamois skin, and washing with water. The prepa- 

 ration was then treated exactly as A, and dried at 110° C. for analysis. 



By long continued contact with water, as in dialysis, the precipi- 

 tated or coagulated myosin is rendered insoluble in dilute salt solu- 

 tions, as noticed by Weyl and others, hence in this preparation it was 

 not possible to purify the substance by reprecipitation. 



The composition of the product (see the accompanying table) is 

 essentially the same as that of the preceding preparation. 



Myosin C, from sheep's muscle. 



This sample of myosin was obtained from fresh mutton in essen- 

 tially the same manner as tlie preceding preparation, viz : by extrac- 

 tion of the thoroughly washed tissue with 15 per cent, ammonium 

 chloride, and separation of the myosin by dialysis. 



On analysis, it was found to possess a somewhat higher percentage 

 of carbon than the two myosins from ox muscle, but in other respects 

 it was identical with them. 



Myosin D, from calfs muscle. 



A solution of myosin was prepared from 3 kilos, of fresh, lean mus- 

 cle from a young calf, by extraction of the thoroughly washed tissue 

 with 15 per cent, ammonium chloride solution. 



From one-half of the filtered fluid, myosin was precipitated by 

 dilution with water and purified by washing with water until chlo- 

 rides were entirely removed. It was then washed with weak alcohol, 

 finally with absolute alcohol and ether, and then dried at 110° C. for 

 analysis (D'). 



From the other half of the ammonium chloride solution, myosin 

 was separated by addition of ammonium chloride in substance. The 

 precipitate was freed from excess of ammonium chloride by addi. 

 tion of just enough water to dissolve the salt, after which the floc- 

 culent myosin was strained off, dissolved in a little water, and precip- 

 itated by pouring the solution into a large volume of water. It was 



