and Casein Peptone. 91 



larger quantity. In water it was readily and completely soluble, 

 the solution giving no turbidity whatever by heat, nor with dilute 

 nitric acid. With cupric sulphate only a very slight turbidity was 

 produced, and with acetic acid and potassium ferrocyanide a corre- 

 spondingly slight turbidity. Its aqueous solution on being saturated 

 with salt and then made slightly acid with acetic acid showed a small 

 flocculent precipitate, doubtless representing the substance which 

 gave the slight turbidity with cupric sulphate and potassium ferro- 

 cyanide, viz : a deuterocaseose. On analysis the following results 

 were obtained : 



ft deuterocaseose from WeyVs casein peptone. 



I. 0-3641 gram substance gave 0*2121 gram H„0 = r47 per cent. 

 II and 0-6087 gram CO^=45-58 per cent. C. 



II. 0-4600 gram substance gave 0-2708 gram H„0 = 6-54 per cent. 

 H and 0-7652 gram CO.^ = 45-36 per cent. C. 



III. 0-3298 gram substance gave 42-2 c. c. N at 13-9° C. and 

 755*4 mm pressure =15-31 per cent. N. 



IV. 0-3418 gram substance gave 43-5 c. c. N at 14-4° C. and 

 756-1 mm pressure =15-31 per cent. N. 



V. 0-3514 gram substance gave 0-0153 gram ash =4-35 per cent. 



VI. 0-3599 gram substance gave 0-0155 gram ash =4-30 per cent. 



Percentage composition of ash- free substance. 



Average. 

 47-50 



_6'79 



16-00 15-84 15-92 



In composition, therefore, as well as in reactions, this body re- 

 sembles the ft deutero described under A, and like it is especially 

 characterized by its exceedingly low percentage of carbon. 



F'rom the first ammonium sulphate precipitate of caseoses, proto- 

 caseose Avas separated by saturation of the aqueous solution of the 

 above precipitate with salt. As in similar precipitates from the 

 preceding digestions, there was considerable heterocaseose present, 

 showing itself as an insoluble residue when the sodium chloride pre- 

 cipitate was dissolved in water for reprecipitation. Purified after 

 the methods previously described, the protocaseose showed the usual 

 reactions characteristic of this body, its aqueous solution growing 

 turbid when heated, giving a precipitate with nitric acid, etc. 



Dried at 110° C. it gave on analysis the following results : 



