88 H. H. Chittenden — Caseoses, Casein Dyspeptone, 



as in the previous digestion. The purified protocaseose showed the 

 same peculiar reactions as protocaseose A., viz: with nitric acid a 

 heavy white precipitate, soluble on warming; with heat alone, a dis- 

 tinct turbidity or coagulum, disappearing as the solution cooled ; and 

 giving with a large amount of water a more or less turbid fluid, from 

 whiich on standing a little gummy matter separated. 



Dried at 110° C. and analyzed, the following results were obtained. 



Protocaseose B. 



I. 0-3470 gram substance gave 0-2192 gram H.,O = 7'02 per cent. H. 



II. 0-2935 gram substance gave 0"1854 gram II„O = 7-01 per cent. 

 H and 0-5779 gram 002 = 53-69 per cent. C. 



III. 0-2952 gram substance gave 0-5853 gram CO^ = bA-0l jDer 

 cent. C. 



lY. 0-2989 gram substance gave 38-6 c. c. N at 13-8° C. and 

 760-1 mm pressure =15*52 per cent. N. 



V. 0-5564 gram substance gave 72-8 c.c. N at 13-1° C. and 

 760*1 mm pressure=15'69 per cent. N. 



VI. 0*3594 gram substance gave 0*0047 gram ash=r30 per cent. 



VII. .0*3523 gram substance gave 0*0045 gram ash = l*27 per 

 cent. 



Percentage composition of ash-free substance. 



Average. 



54-39 54-58 



7-10 

 15*71 15*90 15-80 



In composition, therefore, as in reactions, this body is apparently 

 identical with protocaseose A. 



In the oi'iginal salt-saturated filtrate from protocaseose, acetic 

 acid produced a heavy, gummy precipitate, which was dissolved in 

 water and purified in the same manner as the corresponding body in 

 digestion A. Like the latter, it was readily and completely soluble 

 in water, the solution giving no turbidity whatever by heat, nor on 

 the addition of either nitric or acetic acid. With cupric sulphate, a 

 heavy precipitate was formed, as also with acetic acid and potassium 

 ferrocyanide. 



Dried at 110" C it gave the following results on analysis: 



Acetic acid precipitate B. 



I. 0*3429 gram substance gave 0-2057 gram H.,0 = 6*66 ])er cent. 

 H and 0*6315 gram CO.,=50*22 per cent. C. 



