84 R. H. Chittenden. — Caseoses, Caaein Dyspeptone^ 



Saturation of the aqueous solution with salt gave a slight turbidity. 

 Cupric sulphate gave a heavy precipitate, soluble in excess. 

 On analysis, the substance gave the following results, which show 

 a composition quite different from that of protocaseose. 



Acetic Acid Precipitate A. 



I. 0*4629 gram substance gave 0-2812 gram H„0=r6'74 per cent. 

 H and 0-8608 gram 00^=50-71 per cent. C. 



II. 0-3277 gram substance gave 0-1977 gram H2O = 6'70 ])er cent. 

 H and 0-GOoO gram CO3=50-34 per cent. C. 



III. 0-4253 gram substance gave 53-1 c. c. N at 130" C. and 761-8 

 mm pressui-e=: 15-01 per cent. N. 



IV. 0-4843 gram substance gave 60-8 c. c. N at 13-0° C. and 

 760-8 mm pressure= 15-07 per cent. N. 



V. 0-3649 gram substance gave 0-0108 gram ash = 2-96 per cent. 



VI. 0-3947 gram substance gave 0*0124 gram ash = 3-14 per cent. 



Percentage composition of ash-free substance. 



Average. 



C 52-30 51-92 .-.- 5S-10 



H 6-96 6-90 6-93 



N 15-48 15-54 .15-51 



As previously stated, the above original precipitate produced by 

 acetic acid was not entirely soluble in water. A small residue 

 remained, which after being washed with water was dissolved in 

 dilute sodium carbonate, and the solution neutralized with dilute 

 hydrochloric acid, without yielding any neutralization precipitate. 

 The solution was then dialyzed, concentrated to a syrup and the 

 substance precipitated by alcohol. It was now found, to a great 

 extent, soluble in water, the solution showing no turbidity by heat 

 and giving no precipitate with nitric acid. Cupric sulphate gave a 

 heavy precipitate, and dilute acetic acid added to the aqueous solu- 

 tion produced quite a heavy precipitate, not readily soluble in excess 

 of the acid. The amount of substance was too small to admit of 

 analysis, and the reactions are hardly sufficient to identify it. It is 

 evidently not heterocaseose, for it is only the acetic acid compound 

 that is insoluble in water, not the caseose substance itself. In many 

 respects it appears like casein dyspeptone, and as this substance 

 is precipitated by saturation of its aqueous solution with ammonium 

 sulphate and not by sodium chloride, the presence of a trace of this 

 body might not be impossible. 



