Antipyrinj and Antifehrin on Proteid Metabolism. 49 



under definite conditions of diet, exercise, etc. Nitrogenous equi- 

 librium was established prior to the experiment, and the following 

 daily diet was strictly adhered to throughout the entire period. 



386 grams meat (beef). 



340 " potatoes. 



227 " wheat bread. 



163 " oat meal (steamed). 



28 " sugar. 



42 " butter. 



120 " milk. 



1040 " water. 



In this experiment, nitrogenous metabolism was measured by deter- 

 mining the urea and uric acid contained in the daily excretion, instead 

 of determining the total nitrogen. This was done in order to ascer- 

 tain whether the drug has any special action on the excretion of uric 

 acid. Urea was determined by Liebig's method, as modified by 

 Pfliiger.* Chlorine was previously determined by fusion with potas- 

 sium nitrate and titration with silver nitrate in the usual manner, and 

 then removed from the solution to be tested for urea, by a standard 

 silver solution. Uric acid was determined by Salkowski's method, 

 and phosphoric acid by titration with a standard uranium solution. 



The accompanying tables give the results of the daily analyses. 

 From these it is evident that under the conditions of this experiment, 

 anti pyrin has a decided inhibitory action on the proteid metabolism 

 of the healthy human organism, as shown by the diminished excre- 

 tion of urea and uric acid when the drug is taken. Antipyrin also 

 tends to diminish the volume of the urinary secretion, this action 

 being very marked in the second series, where comparativelv laro-e 

 amounts of antipyrin were administered. As regards the excretion 

 of phosphoric acid and of chlorine, nothing definite can be said. The 

 raoi*e important changes produced by the antipyrin are shown in the 

 following table of average daily results. 



Urea. Uric acid. Total P2O5. Volume. Sp.Grr. 



grams. gram. grams. c. c. 



Normal period 41-806 0-586 3-185 951 1028 



First antipyrin period 38-375 0-556 3-026 848 1029 



First after period- 42-030 0-575 2-929 929 1028 



Second antipyrin period.. 40*854 0-472 2-941 822 1031 



Second after period 44-220 0-537 2-923 957 1028 



* Pfliiger's Archlv fiir Physiologie, vol. xxi, p. 248. 

 TuANs. Conn. Acad., Vol. VIII. 7 Nov., 1888 



