34 Chittenden and Hart — Elastin and the Elastose Sodies. 



Horbaczevv ski's elastin peptone is described as not giving a precipi- 

 tate with acetic acid and neutral salt. Deuteroelastose, likewise, 

 gives no precipitate with acetic acid and salt, except when the solu- 

 tion is saturated or nearly saturated with the salt. 



Another preparation of deuteroelastose was separated from a 

 pepsin-hydrochloric acid digestion of elastin B. Its chemical com- 

 position is shown in the accompanying table, from which it is seen 

 to differ somewhat from deutero A, but like It has a lower content of 

 carbon than the proto body. 



Analysis of Deuteroelastose B. 



Percentage composition of ash-free substance. 



16-81 



16-90 



Average. 



53-11 



7-08 



16-85 



22-96 



100-00 



Digestion of dastin u'lth pancreatic juice. 



A strong solution of pure trypsin was prepared, according to 

 Kiihne's method,* from 100 grams of dried ox pancreas and added to 

 150 grams of powdered elastin B in 2 litres of 0-5 per cent, sodium 

 carbonate. The mixture M'as warmed at 40° C. for four or five days, 

 a little thymol being added to prevent putrefaction. At the end of 

 this time, the elastin was nearly all dissolved and the filtered fluid 

 on being tested gave the ordinary elastose reactions. On saturation 

 of a portion of the neutralized fluid with ammonium sulphate, a 

 heavy gummy precipitate was obtained, and in the filtrate no trace 

 of peptone I'eaction could be found. 



* Kiihoe and Chittenden, Zeitschrift fiir Biologie, Band xii, p. 196. 



