32 Chittenden and Hart — Elastin and the Elastose Bodies. 



produce a noticeable turbidity, as does also alcohol, and acetic acid 

 and sodium chloride. Moreover, the aqueous solution becomes some- 

 what clouded on heating, clearing up again as the solution cools. 

 Hence, the reactions would indicate a preponderance of deuteroelas- 

 tose, while the composition points to an excess of the proto body. 



Analysis of the Second Ammonium Sulphate Precipitate. 



c 



H 



N 

 O 



Percentage composition of ash-free substance. 



Average. 



54-14 53-93 5^-03 



7-30 7-22 .... -... 7-26 

 16-92 16-89 lG-90 



S2-S1 



100-00 



We must conclude, then, that this second gummy precipitate is sim- 

 ply a residue of the mixed elastoscs not at first precipitated, perhaps 

 from a lack of complete saturation with ammonium sulphate. Past 

 experience, however, has shown that deuteroproteose is not as quickly 

 or completely precipitated witli ammonium sulphate as the other 

 proteoses. 



From the first ammonium sulphate precipitate, a large amount of 

 protoelastose (A 3) was separated, having all of the reactions charac- 

 teristic of this body, and the composition, when dried at 110° C, 

 shown in the accompanying table. 



A larger amount of deuteroelastose was separated from this diges- 

 tion than in the preceding one, and after purification by the use of 

 methods already described, a portion was dried at 110° C. for analy- 

 sis. Its composition is shown in the accompanying table. Like the 

 deuteroelastose formed by dilute hydrochloric acid, it contains a 

 lower percentage of carbon than elastin or the proto body, while its 

 content of nitrogen is higher than that of elastin. Its reactions were 

 the same as the deutero previously obtained. 



