28 Chittenden and Hart — Elastin and the Elastose Bodies. 



compared. Deuteroelastose was readily soluble both in cold and hot 

 water, and on heating a cold saturated solution only a slight turbidity 

 was seen which, as in the proto reaction, disappeared when the solu- 

 tion cooled. This turbidity was no doubt due to the presence of a 

 small amount of protoelastose, for later on we obtained a purer deu- 

 tero body having nearly all of the reactions of the present preparation 

 in which, however, the heat reaction was absent. 



Analysis of Deuteroelastose. A 1. 



16-76 



16-64 



Percentage composition of ash-free substance. 



Average. 



53-26 



T12 



16-70 



22-92 



100-00 



The deutero body, likewise, gave no precipitate whatever with 

 the concentrated mineral acids, nor with acetic acid and potassium 

 ferrocyanide. Alcohol gave no precipitate and salt-saturated 30 per 

 cent, acetic acid, when added to an aqueous solution of the substance, 

 also gave no precipitate. 



Ferric chloride gave a slight turbidity, but no precipitate. With 

 the other reactions of protoelastose, the deutero body showed close 

 agreement. Pure deuteroelastose, formed later on by the action of 

 pepsin -hydrochloric acid, gave no precipitate whatever with concen- 

 trated potassium hydroxide. This reaction, Avhich is quite character- 

 istic of protoelastose, was fairly distinct in the deutero body first 

 described, which fact, coupled with the turbidity produced by heat, 

 may be considered good evidence that the deuteroelastose was not 

 perfectly pure. 



