Chittenden and Hart — Elastiu and the Elastose Bodies. 25 



Protoelastose. 



The ammonium sulphate precipitate of elastoses, after being washed 

 as described above, was dissolved in water, the solution made quite 

 neutral by addition of a little sodium carbonate and then saturated 

 with crystals of rock salt. A heavy gummy precipitate resulted, 

 which by analogy should consist of what we may call protoelastose. 



This precipitate, after being washed with saturated salt solution, 

 was dissolved in water and reprecipitated by salt, this process being 

 repeated three times. The final sodium chloride precipitate was dis- 

 solved in water, dialyzed until all chlorine was removed from 

 the solution, concentrated and precipitated with 95 per cent, alcohol. 

 Apparently, only a portion of the substance was precipitated by 

 alcohol, so the alcoholic filtrate was evaporated and the residue care- 

 fully dried. Further examination, however, showed that both pro- 

 ducts were identical, alcohol simply precij^itating a portion of the 

 substance. 



After being dried at 110° C. until of constant weight, having then 

 the form of a brown powder, both ])roducts were analyzed Avith th^ 

 results shown in the accompanying tables. 



Analysis of Protoelastose (A1) Precipitated by Alcohol. 



Percentage composition of ash-free substance. 



16-75 



16-56 



Average. 



54-39 



7-17 



16-65 



21-79 



Trans. Conn. Acad., Vul. VIII. 



100-00 



Nov., 1888. 



