20 Chitten(le7i and Hart — Elastin and the Elastose Bodies. 



2400 grams. The mass was boiled for four days in water frequently 

 changed, after which it was soaked for 45 hours in 8 litres of 1 per 

 cent, potassium hydroxide, and then boiled with the solution for 4 

 hours. The tissue was freed from alkali by thorough washing with 

 water, and was then boiled with water, frequently changed, for 16 

 hours. The potassium hydroxide solution on neutralization, gave a 

 heavy precipitate slightly soluble in excess of 0-4 per cent, hydro- 

 chloric acid, easily soluble in strong acid. The addition of acid in 

 excess caused a strong odor of hydrogen sulphide to be given off. 



After boiling the tissue a second time with water, it was placed in 8 

 litres of 10 per cent, acetic acid and warmed for an hour and a half, 

 after which it was allowed to stand in the cold for 16 hours. It was 

 then boiled for 4 hours in the same acetic acid solution, and after- 

 wards washed thoroughly with water. The tissue was then placed 

 in 10 litres of 5 per cent, hydrochloric acid and allowed to soak for 

 some time in the cold, after which it was washed in running water 

 and macerated in water, frequently changed, for 24 hours to take out 

 all traces of the acid. After this treatment, the tissue was boiled 

 with successive portions of water for about 45 hours, when it was 

 placed in a large volume of 95 per cent, alcohol and allowed to re- 

 main for several days. It was afterwards boiled in alcohol for about 

 15 hours and then placed in an excess of ether, after which it was ex- 

 tracted with warm ether for two days. 



Analysis of Elastin A. 



Percentage composition of ash-free substance . 



C 

 H 



N 

 O 



54-26 

 7-24 



54-22 

 7-30 



16-74 



16-76 



16-62 



Average. 



54- 24 



7-^7 



16-70 



21-79 



100-00 



