VIII. — Myosinoses, By W. Kuhne and R. H. Chittenden. 



Of the primary digestion jDroducts of the various proteids, the 

 albumoses from fibrin * and egg albumin f have been more or less 

 carefully examined and analyzed, as also the globuloses,J the case- 

 oses,§ and elastinoses. || Further, the vitelloses ^ have likewise been 

 prepared from crystalline phyto-vitellin and their properties ascer- 

 tained. With the hope of gradually completing the list we have 

 undertaken a study of the primary digestion products of myosin, the 

 results of which we now present. 



For the preparation of myosin, large quantities of finely divided 

 ox muscle were extracted with cold water until the fluid no longer 

 gave reaction for albumin, after which the tissue was placed in an 

 excess of a 15 per cent, ammonium chloride solution, and the myosin 

 ultimately precipitated from the filtered fluid by dialysis.** The ex- 

 traction of myosin with ammonium chloride, after the method of 

 Danilewsky,ff is far better in every way than the older method of 

 extraction with 10 per cent, sodium chloride, since myosin is dis- 

 solved more rapidly and completely by the ammonium salt, even 

 from coarsely divided muscle, and at the same time forms a more 

 easily filterable fluid. Further, the advantage of precipitating the 

 myosin by dialysis in running water, instead of pouring the ammo- 

 nium chloride solution into a large excess of water, consists in the 

 avoidance of the large volumes of fluid necessary in the preparation 

 of such a large quantity of myosin, while at the same time the my- 

 osin is obtained equally free from soluble salts. At the best, how- 

 ever, the preparation of such a quantity of myosin as was needed in 

 the present investigation involved a large amount of labor and a 

 comparatively low temperature, hence compelling us to take the 

 winter season for the work. The united products obtained in this 

 manner were treated ultimately with dilute alcohol, whereby the 

 semi-gelatinous mass was naturally more or less shrunken, and finally 

 with ether. 



* Zeitschrift fiir Biologic, Band xix, p. 159 and Band xx, p. 11. 



f Studies from Laboratory of Physiological Ciiemistry, Yale University, vol. ii, p. 126. 



J Zeitschrift fiir Biologic, Band xxii, p. 409. 



§ This volume, p. 66. ||This volume, p. 19. 



^ Ueber vitellogen, by Dr. R. Neumcister. Zeitschrift fiir Biologic. Baad xxiii, p. 2. 



** See the preceding article. 



ff Zeitschrift fiir Physiologische Chemie, Band v, p. 158. 



