140 W. Kiihne and R. H. Chittenden — Myosinoses. 



A sample of the product, carefully dried at 110° C. until of con- 

 stant weight, yielded the following results on analysis. 



Myosm. 



I. 0-3665 gram substance gave 0'2313 gram H„0 = 7*01 percent. 

 H and 0-7053 gram C0^= 52-48 per cent. C. 



II. 0-4900 gram substance gave 0*9404 gram 00.^= 52-48 per cent. 

 C. 



III. 0-2913 gram substance gave 0-1868 gram \{JJ=z1-Vi per 

 cent. H and 0-5596 gram CO^ = 52-38 per cent. C. 



IV. 0-7740 gram substance gave 109-1 c.c. N at 15-4° C. and 759-8 

 mm pressure = 16-73 per cent. N. 



V. 0-5013 gram substance gave 70-6 c.c. N at 14-7° C. and 757-8 

 mm pressure = 16-72 per cent. N. 



VI. 0-7524 gram substance gave 105-9 c.c. N- at 15-0° C. and 760-7 

 mm pressure = 16-77 per cent. N. 



VII. 0-6629 gram substance gave 0-0043 gram ash =r 0-65 per 

 cent. 



VIII. 0*6741 gram substance gave 0-0046 gram ash = 0-68 per 

 cent. 



IX. The ash from 1-3370 grams substance gave 0-002 gram BaSO^ 

 = 0-02 per cent. S. 



X. 0-4967 gram substance gave by fusion with koh + KNO3, after 

 Hammarsten's method, 0-0458 gram BaSO^:=r26 per cent. S; de- 

 ducting 0-02 per cent. S of ash = 1*24 per cent. 



XI. 0-5992 gram substance gave after fusion with koii + knOj 

 0-0552 gram BaSO^=l-28 percent. S; deducting 0-02 per cent.=i 1-26 

 per cent. 



Percentage composition of ash-free myosin. 



Average. 



- 52-79 



.... 712 



16-86 16-83 16-88 16-86 



- 1-35 1-27 1-26 



21-97 



100-00 



The ash consisted almost entirely of calcium phosphate. 



Digestion of Myosin. 



The myosin purified as described with alcohol and ether, proved 

 so resistant to the action of pepsin-hydrochloric acid, tliat its diges- 



