142 W. KiXhiie and R. H. Chittenden — Myosinoses. 



would give any further turbidity. From this it is evident that the 

 neutral digestive fluid is not completely precipitated by simple satu- 

 ration with sodium chloride, but that complete precipitation is 

 reached only when the absolute quantity of salt stands in a certain 

 proportion to the proteoses present. 



After this separation of the first portion of the myosinoses, which 

 would naturally consist of proto, hetero, and dysmyosinose, the re- 

 mainder were precipitated first with salt-saturated 30 per cent, 

 acetic acid and then, after removal of the sodium chloride by dialy- 

 sis, with neutral ammonium sulphate. In the solution i-emaining 

 from this last precipitation, peptone was detected by the biuret re- 

 action. 



Protomyosinose. 



The sodium chloride precipitate, after thorough washing with 

 saturated salt solution, dissolved almost entirely on being rubbed up 

 with water. 



What did remain undissolved, showed the reactions of dysalbu- 

 mose. it was insoluble in water and in salt solutions of all strengths, 

 but easily soluble in 0*1 per cent, hydrochloric acid, much more diffi- 

 cultly soluble in 0*5 per cent, sodium carbonate, by neutralization 

 only partially precipitated and gave the biuret reaction. From the 

 filtrate of the neutralized hydrochloric acid solution, some hetero- 

 myosinosc in the form of a flocculent precipitate was obtained by 

 dialysis. This hetero body was soluble in dilute sodium chloride 

 solutions, insoluble in water, precipitable by salt in substance, and 

 after the manner of the albumoses gave a precipitate with nitric acid 

 in tlie cold, which disappeared as the mixture was warmed, reappear- 

 ing as the solution cooled. This heteromyosinose, formed from dys- 

 myosinose by a process of retrogression, amounted to considerable ; 

 about 1'5 grams. 



Reactions of Protomyosinose. 



In order to purify protomyosinose the aqueous solution of the 

 substance was freed from sodium chloride by dialysis, by which only 

 traces of heteromyosinose separated, and the solution evaporated to a 

 thin syrup. The fluid was filtered from a slight flocculent albumin- 

 like prccii)itate insoluble in hot water, somewhat further concen- 

 trated and the pure myosinose separated by alcohol. After washing 

 with alcohol and ether it appeared as a light, white powder. The 

 yield amounted to about ten grams. 



