Wl Kilhne and R. H. Chittenden — Myosinoses. 143 



The reactions of protomyosinose agree in general with those of 

 protoalbumose, but with one important difference, viz : that an 

 aqueous solution of the former, free from salt, of whatever concen- 

 tration, is not rendered turbid by nitric acid. Addition of even a 

 little sodium chloride to the acid fluid, however, is sufficient to cause 

 a heavy precipitate, soluble as the mixture is warmed but reappear- 

 ing as the solution cools. The precipitate is also soluble in an ex- 

 cess of the acid in the cold. 



Protomyosinose is readily soluble in distilled w^ater, the solution 

 showing a weak, but unquestionably alkaline reaction. In this solu- 

 tion, as well as in a solution rendered acid by acetic acid, cupric sul- 

 phate produces a heavy turbidity, which on boiling almost entirely 

 disappears. Acetic acid and potassium ferrocyanide produce a heavy 

 precipitate, insoluble in glacial acetic acid. Neutral lead acetate 

 gives no precipitate. Basic lead acetate and mercuric chloride 

 both produce a heavy turbidity. An aqueous solution of the 

 myosinose boiled with sodium hydroxide and lead acetate is colored 

 deep bi'own or black; with sodium hydroxide and cupric sulphate a 

 beautiful red. Concentrated sodium hydroxide, as in protoelastose, 

 produces a heavy flocculent, gelatinous precipitate. By saturation 

 of an aqueous solution of protomyosinose with sodium chloride, only 

 a portion of the substance is precipitated ; the portion remaining dis- 

 solved is precipitable by acid. 



The composition of the substance, dried at 110° C. until of con- 

 stant weight, is shown by the following analysis. 



Protomyosinose. 



I. 0-3'722 gram substance gave 0-2387 gram H^O = 7"12 per cent. 

 H and 0-7090 gram C0^= 51-95 per cent. C. 



II. 0-4239 gram substance gave 0-2688 gram H^O = 7-05 per cent. 

 H and 0-8048 gram CO^= 51-77 per cent. C. 



III. 0-3319 gram substance gave 0-2138 gram IT.^0 = 7-15 per cent. 

 H and 0-6308 gram C0^= 51-83 per cent. C. 



IV. 0-4798 gram substance gave 67-5 c. c. N at 14-8° C. and 762-1 

 mm pressure = 16*79 per cent. N, 



V. 0-5663 gram substance gave 78-3 c. c. N at 15-1° V. and 768-2 

 mm pressure = 16-64 per cent. N. 



VI. 0-4429 gram substance gave 62-7 c. c. N at 15-8° C. and 758-2 

 mm pressure = 16-77 per cent. N. 



VII. (»-5494 gi-am substance gave 0-0062 gram ash = 1-13 percent. 



VIII. 0-7080 gram substance gave 0-0081 gram ash=l-14 per cent. 



