1 44 W. JKiihne and R. S. Chittenden — Myosinoses. 



IX. The ash from 1-2574 grams substance gave 0-0132 gram BaSO^ 

 = 0-14 per cent. S, 



X. 0-4183 gram substance gave after fusion with koh + kno^ 

 0-0450 gram BaSO^=l-47 per cent. S ; after deducting 0-14 per cent. 

 S of the ash = 1-33 per cent. 



XI. 0-3963 gram substance gave after fusion witli koh 4- kno^ 

 0-0411 gram BaSO^=: 1-42 per cent. S; deducting 0*14 per cent.rr 

 1-28 per cent. 



Percentage composition of ash-free protomyosinose. 



Average. 



--- .. .--. o3-4a 



-... 7-17 



16-98 16-83 16-96 16-93 



1-35 1-80 1-33 

 32-16 



100-00 



The ash contained only calcium phosphate and sulphate, with a 

 little ferric oxide. 



Deuteromyosinose. 



This myosinose, as we have before mentioned, was obtained mainly 

 by saturation with ammonium sulphate. After removal of the 

 greater portion of protomyosinose by saturation with salt as de- 

 scribed, deuteromyosinose was in part precipitated by salt-saturated 

 acetic acid, but this precipitate could not be used on account of the 

 large amount of proto body precipitated with it, as shown by the 

 cupric sulphate reaction. This method of treatment, however, 

 although necessitating the loss of considerable deuteromyosinose, 

 enabled us to remove the proto body completely and thus ensure a 

 pure specimen of deuteromyosinose on treatment of the filtrate with 

 ammonium sulphate. Naturally, before saturating the fluid with the 

 ammonium salt, the sodium chloride was removed by dialysis. The 

 myosinose thus precipitated by saturation with ammonium sulphate 

 was dissolved in water and dialyzed until the sulphate was entirely 

 removed, or to such an extent that the fluid gave only the slightest 

 turbidity with barium chloride even on long standing. We hastened 

 the removal of the sulphate by repeated evaporation and renewed 

 dialysis of the concentrated fluid. The substance was finally pre- 

 cipitated from the suitably concentrated fluid, as a white powder, by 

 alcohol and washed with alcohol and ether. It weighed forty-five 

 grams. 



