W. Kuhne and It. H. Chittenden — Myosinoses. 145 



Reactions of deuteromyosinose. 



Like the preceding myosinose, this body also reacts alkaline in an 

 aqueous solution and this property exercises more or less of an influ- 

 ence on certain of its reactions. In order to convince ourselves of the 

 absence of traces of either proto or heteromyosinose, we paid particu- 

 lar attention to the behavior of the deutero body towards the cupric 

 sulphate reaction, which according to Neumeister's observations is a 

 decisive test on this point. A fairly concentrated solution of our 

 preparation was not rendered turbid by cupric sulj^hate in the cold, 

 but after boiling and then cooling the mixture, a slight turbidity 

 appeared. Solutions of the substance so concentrated as to be 

 almost syrupy, gave a slight turbidity at once, the turbidity disap- 

 pearing when the solution was heated and reappearing as the fluid 

 cooled. By partial neutralization of the alkalinity of the myosinose 

 solution, leaving the fluid, however, still alkaline to delicate test 

 papers, precipitation by cupric sulphate was entirely prevented. As 

 protomyosinose is precipitated by the copper salt equally well in an 

 acid fluid, we are led to consider our deuteromyosinose entirely free 

 from this impurity. 



In general, deuteromyosinose shows much the same reactions as 

 deuteroalbumose, but is somewhat different from the latter in that it 

 is more difficultly precipitable. Acetic acid and nitric acid produce 

 a precipitate only after addition of sodium chloride to saturation. 

 Acetic acid and potassium ferrocyanide give a decided turbidity, not 

 soluble in glacial aectic acid. Basic lead acetate and mercuric 

 chloride both produce a precipitate, insoluble in excess of the rea- 

 gent. Cold nitric acid quickly produces an intense yellow color. 

 The biuret reaction comes out distinctly, but on boiling the my- 

 osinose with sodium hydroxide and lead acetate only a faint brown- 

 ing of the fluid is obtained. 



The composition of the substance, dried at 110° C, is shown by 

 the following analysis : 



Deuteromyosinose. 



I. 0-2121 gram substance gave 0-1394 gram 11^0= 7-30 per cent. 

 H and 0-3896 gram C0„=: 50*09 per cent. C. 



II. 0-2115 gram substance gave 0-1390 gram H^O = Y'30 per cent. 

 H and 0-3891 gram 00^= 50-12 per cent. C. 



III. 0-3830 gram substance gave 53-6 c. c. N at 15*0° C. and 

 762-5 mm pressure = 16-71 per cent. N. 



Trans. Conn. Acad., Vol. VIII. 19 Dec, 1888. 



