146 W. Kilhne and It. H. Chittenden — Myosinoses. 



ly. 0-2940 gram substance gave 41*4 c. c. N at 15-7° C. and 759-V 

 mm pressure = 16*'72 per cent. N. 



V. 0-4672 gram substance gave 0-0080 gram ash =1-77 per cent. 



VI. 0-5462 gram substance gave 0-0094 gram ash = 1-72 per 

 cent. 



VII. The ash from r0034 grams substance gave 0-0116 gram 

 BaSO^=0-15 per cent. S. 



VIII. 0-3343 gram substance gave after fusion with koh + KXOg 

 0-0315 gram BaSO^= 1-29 per cent. S ; after deducting 0-15 percent. 

 S of the ash = 1-14 per cent. 



IX. 0-4050 gram substance gave after fusion with koh + kno 

 0-0420 gram BaSO^rr: 1-42 per cent. S; deducting 0-15 per cent.:= 

 1-27 per cent. 



Percentage composition of ash-free deuteromyosinose. 



Average. 



C 50-95 50-98 .... ..-. 5097 



H 7-42 7-42 • .... 7-43 



N 17-00 17-01 17-00 



S .... -. .... 1-16 1-28 1-22 



O 23-.39 



100-00 

 The ash consisted only of calcium pliospiiate and sulphate, with 

 some oxide of iron. 



This is as far as we have been able at present to carry our study of 

 the myosinoses, since hetero and dysmyosinose appear to have been 

 oresent in the digestions only in very small quantity. Myosin 

 purified by alcohol, as was the preparation employed by us, is so diffi- 

 cultly digestible that it is attacked only by the most energetic pepsin 

 mixture, and this has the disadvantage of rapidly converting hetero 

 and protoproteose, which according to Neumeister's * investigations 

 are formed in the beginning of digestion, into the deutero body ; con- 

 sequently in the present instance we could expect a large amount only 

 of deuteromyosinose. Corresponding with this view, we obtained for 

 45 grams of deuteromyosinose, only 10 grams of protomyosinose and 

 but 3 grams of hetero and dysmyosinose. Further, the unavoidable 

 loss attending the separation of these bodies was probably greater 

 with deuteromyosinose than with the others. Portions of the fluid 

 from the first and second digestion, tested before they were united, 

 showed also a difference in that the fluid from the first digestion con- 



* Loc. cit. 



