110 



Chittenden and Blake — Influence of Arsenic 



Experiment III, 



Action of arsenic on a rabbit. 



Period of dosing. 

 May 25-31, 02 grain AsoOs daily. 



June 1-15, 0-3 " " " 



" 16-23, 0-4 " " " 



9'1 grains. 



A, control rabbit. B, arsenic rabbit. 



Body weight May 25, 1644 grams. 1502 grams. 



" " June 24, 16l6 " 1586 



— 28 +84 



Weight of liver June 24, 61'360 grams. 46'760 grams. 



Determination of fat in liver. 



A, control, 15'320 grams liver gave 0*4126 gram fat, := 2"69 per cent. 



B, arsenic, 14-435 " " " 0-2888 " " = 2-00 " 



—0*69 per cent. 

 Determination of glycogen and sugar. 



A. B. 



Weight of liver used, 45'940 grams. 32-325 grams. 



Glycogen A,* control. 



Volume used Equivaleut Equivalent Total Per 



lor reduction. Weight Cu. in dextrose. in glycogen. amount. cent. 



25 c. c. 0-2036 gram. 0-1045 gram. 0-0940 gram. 1-8800 grams. 4-13 



25 0-2027 01041 0-0937 1-8740 4-07 



Glycogen B, arsenic. 



25 c. c. 0-5230 gram. 0-2503 gram. 0*2252 gram. 1-8016 grams. 5-57 



25 0-5223 0-2500 0'2250 1-8000 5-54 



Sugar A, control. 



25 0. c. 0-0177 gram. 0-0098 gram. 0-0784 gram. 0-lt 



25 0-0173 0-0097 0-0776 0-16 



Sugar B, arsenic. 



25 c. c. 0-0254 gram. 0-0137 gram. 0-1096 gram. 0-34 



25 0-0232 0-0126 0-1008 0-31 



100 grams of muscle tissue yielded 0-3 milligram of J.5. 



Here, we find under the influence of the arsenic, an apparent gain, 

 though slight, in body weight, an evident diminution in the weight 

 of the liver, together with a diminished percentage of liver fat. Ap- 

 parently, however, the amount of liver glycogen and sugar are some- 

 what increased. The liver showed no approach to fatty degeneration 

 on microscopic examination. 



* The glycogen solution after boiling with acid, etc., was made up to 500 c. c. instead 

 of 200 c. c. 



