2g8 LESTER W. SHARP 



Flemming's stronger solution : 



1 % chromic acid 4.6 ce. 



2 % osmic acid 12 ce. 

 Glacial acetic acid 3 ce. 



Benda's modification of the above : 



1 °/o chromic acid 43 ce. 



2 % osmic acid 12 ce. 

 Glacial acetic acid 6 drops. 



A weaker chromo-acetic-osmie solution : 



Chromic acid 1/4 gm. 



Glacial acetic acid i ce. 



Water 100 ce. 



I % osmic acid 3o drops. 



Merkel's solution : 



1.4 % solution chromic acid ) „ 



, . , . . , , • 1 Equal parts. 



1.4 °/o solution platinic chlonde > 



Bouin's solution : 



40 °lo Formalin 

 Picric acid (sat. sol.) 

 Glacial acetic acid 



Flemming's weaker solution : 



I 0/0 chromic acid 



I % acetic acid 



Water 



I °/o osmic acid 



The effeets of each of thèse solutions are briefly summarized below. 



Flemming's stronger solution fixes well only the peripheral cells of 

 the root, five or six layers at the most. In the interior cells the structures 

 appear to swell and flow together, an effect due in ail probability to the 

 large proportion (5 %) of acetic acid, which, on account of the more rapid 

 pénétration of this acid, becomes even greater by the time the solution has 

 reached the central tissue. Thas the inner cells are worthless for critical 



