3o4 LESTER W SHARP 



most rapidly from the peripheral portion, as would be expected, since it is 

 first attacked by the decolorizing agent (the ^ Spiegelfarbung - ot Alfred 

 Fischer, gg). The axial région loses color before the outer portion is com- 

 pletely decolorized because of its thinner consistency. In fig. F is shown 

 a slightl}^ later stage. The chromosome at the right has been eut longitu- 

 dinally and shows the less dense axial portion in side view, while the 

 lower ends of two of the other chromosomes show the efifect due to an 

 oblique eut. 



We désire to lay emphasis upon the fact that in Vicia this irregular 

 differentiation does not yet represent a real vacuolization of the chromo- 

 some, nor its true constitution. The rétention of the stain only in certain 

 parts of the chromosomes of fig. B-F" is largely due to the irregular action 

 of the reagents, for in the same préparation from which fig. C and D are 

 taken we find the ^ Spiegelfarbung - of Alfred Fischer, fig. E and F. 

 It is probable, howewer, that the aspects are due in part to the beginning 

 of the transformation of the chromatic material, which comes to its fuU ex- 

 pression at the time of the telophasic vacuolization. As for the appearance 

 of the wholly pale régions, it seems to be due simply to a differentiation of 

 a denser and consequently more heavily staining outer portion, the inner 

 portion remaining less dense and consequently lighter; comparative study 

 shows that ail the substance is chromatic. 



On normally stained material a light line may nearly always be seen 

 running along the center of each chromosome, as certain other writers hâve 

 described, but in our préparations this is due solely to phenomena of re- 

 fraction. On the much larger chromosomes of Trillium, on the other hand, 

 Grégoire and Wygaerts (o3) show that the vacuolization begins much 

 earlier, even at the metaphase. The writer has been privileged to see the 

 excellent préparations upon which this statement is based. 



Telophase. 



After remaining tightly pressed together for a short time the chromo- 

 somes begin to separate. As they do so they adhère to one another at va- 

 rious points where the substance then becomes drawn out to form anasto- 

 moses, FIG. 34, 35. According to Boveri (04) the anastomoses are sent 

 out from the chromosomes after the manner of pseudopodia. A similar 

 opinion is held by Gates (12) and by Lundegâkdh. Other investigators, 



