THE NEWTS STOMACH DURING DIGESTION 407 



The sections were stained by immersion in various dyes, préférence 

 being given to î\Iann"s " Melhyl-bliie-Eosine long method and to M. Hei- 

 denhain's"" Iron Alitm Hœmatt)xylin, both long and substractive methods. 

 Other methods used were Toliiidin-blue and Eosine which gives good but 

 fugitive results ; it is indeed an excellent method for cytological study where 

 the work can be done rapidly, but becomes useless where long intervais of 

 time must elapse before the work can be completed. For the study of mu- 

 cigen in the surface cells I devised the following method. After dissolving 

 out the corrosive with a solution of iodine in iodide of potassium and 

 vvashing this out with alcohol and water the sections were treated for ten 

 minutes with a 1/2 0/0 watery solution of méthylène blue, patent B. Then 

 washed in running water after which a 0,6 0/0 solution of potassium bichro- 

 mate in water was poured over the slide and allowed to remain till the sec- 

 tions assumed a violet colour, about half a minute; then washed in water, 

 dehydrated in absolute alcohol (some blue comes out) and cleared in xylol 

 or inspissated turpentine and finally mounted in balsam. 



By this method the zymogen granules in the surface cell and in thèse 

 of the pyloric glands stain violet and can be readily studied, whilst the large 

 mucous cells of the necks of the fundus glands remain colourless. Chromatin 

 in nuclei ofall cells stains bright blue and the nucleoli remain unstained 

 whilst the zymogen granules of the fundus gland cells stain green or greenish 

 blue. Thèse appearances are however very fugitive lasting only a week or se. 

 Triacid, methyl violet 5 B and methyl violet 6 B, were also used. 



The préparations wei"e studied with the aid of a 1/12 oil immersion lens 

 of Leitz and No 3 eyepiece. The illuminant was in ail cases a paraffin lamp 

 which gave a somewhat yellow light. The drawings were made with a Na- 

 CHET caméra lucida and ail measurements with a Zeiss eyepiece micrometer. 

 The photomicrographs were taken with a magnification of 600 linear. 



The cells in three régions of the stomach were examined ; namely : 



1 . The glands of the œsophageal end. 



2. The glands of the pyloric end. 



3. The glands of the narrow zone between 1 and 2. 



The shape and arrangement of the cells in thèse différent glands hâve 

 been sufficiently described by Langley and others and require no répétition 

 hère. 



In the présent communication I shall confine my remarks chiefiy to 

 the cells of the oxyntic glands and when colour is mentioned it refers to 

 spécimens stained with methyl-blue-eosine. 



