THE NEWTS STOMACH DU RING DIGESTION 41 1 



size of nuclei 8,87 X 1 1,''^8 i^- i. e. they are considerably smaller than in the 

 preceding spécimens. 



The lanthanin granules are very numerous, of small size and densly 

 crowded togetlier, to such an extent that it is difficult to distinguish other 

 nuclear structures. They stain deep dull grey-blue and impart by their num- 

 ber and deep coloration a misty appearance to the nuclei, Plate III, fig.3. 



The nuclcoli are often of immense size, and maybeseen passingthrough 

 the nuclear wall which closes behind them, when in the nucleus they lie in 

 a tiny vacuole free frorn lanthanin granules. They may measure as much 

 as 3,93 X 3,9 i^ or 2,2 X 4, 1, or i ,6 X 4,83 showing that they vary in shape 

 from nearly rounded to elongated rod like bodies. Occasionally two of thèse 

 big nucleoli may be seen in the same nucleus, but as a rule there is only 

 one of such size the others being very much smaller. 



At this stage therefore the cells hâve already recovered considerably 

 from their previous exhausted condition and hâve manufactured many new 

 zymogen granules, but the nuclei exhibit every sign of great exhaustion, 

 Nevertheless mitotic figures may be seen in a few of them. 



A few acini exhibit a more advanced stage being intermediate between 

 thèse just described and thèse of the 68 hours newt, the nuclei are less 

 cloudy and wrinkled and the lanthanin tends to stain of a violet hue in a few 

 cases. 



68 Hours afterfood. — Plate I, fig 5. 



On opening the stomach of this animal the worm was found to hâve 

 been dissolved but the organ still contained a quantity of débris and mucus. 



The gland lumen is now small, the cells large and crowded with big 

 zymogen granules, nearly ail of one size, averaging 1,5 jj- — 1,7 :j^ in diame- 

 ter. The protoplasm has recovered its pale blue tint, but does not stain so 

 vividly as in the fasting animal. 



The nuclei are very conspicuous owing to their deep blue colour, due 

 not to chromatin,_ which is practicall}^ invisible but to the multitude of fine 

 deeply stained lanthanin granules, that completely fill them. When a view 

 of the chromatin is obtainable, it is seen to be scant}-, but of a deeper blue 

 colour than in the previous section. 



The average size of the nucleus is 10,57 X '2,54 \>., it is therefore be- 

 coming larger, probably swelling up by absorption of material from the pro- 

 toplasm and this agrées well with the disappearance of the wrinkles from its 

 surface, so obvious in the foregoing préparation. 



52 



