4l6 E. WACE CARLIER 



The nucleoli are still of large size, oval in shape and stain venous red. 

 They are rendered more conspicuous by the comparative absence of chro- 

 matin, indeed some nuclei appear almost devoid of that material except 

 round the nuclear envelope. Extrusion of nucleoli is progressing and many 

 cells exhibit mitotic figures. 



Near the pyloric end where the stage appears less advanced the lan- 

 thanin granules are more numerous as are also the karyosomes. 



3 Hoiirs a/ter food. ~ Plate I, fig. li. 



The lumen is very wide, the cells being often reduced to narrow strips 

 containing a few zymogen granules, that are mostly confined to their free 

 extremities. The granules measure about 1,1 i^- in diameter. The protoplasm 

 is very pale grey or even colourless, perhaps tinted with pink. The cell 

 envelope remains unchanged. 



The nuclei are much wrinkled measuring 9,2 [^ X 10,82 [j. and ccntain 

 very little chromatin which is stained of a decided red tint; rendering it 

 difficult to distinguish from the nucleoli, Plate III, fig. 8. 



This change of reaction of the nuclear chromatin was foreshadowed 

 in a few cells of the preceding spécimen and is remarkable as it must indi- 

 cate a change in the chemical character of the chromatin. This affinity for 

 acid dyes is also présent in the chromatin of the head of the spermatozoon 

 and was looked upon b}^ Geddes and Thompson as katabolic in character. 

 In this case also it will be remarked that the change of reaction of the chro- 

 matin takes place at about the time of cell exhaustion or a little before that 

 time. The lanthanin granules are very pale and almost invisible. Extrusion 

 of nucleoli is still going on but mitosis seems arrested. 



\ Hours, aflev food. — Plate I, fig. 12. — Plate II, fig. 13. 



The lumen is large and the cells very small with only a few granules 

 of zymogen in them, the granules vary in size some cells containing only 

 médium sized granules, other exhibiting some smaller ones and some hâve 

 only one or two granules at most. Most of the cells however contain a group 

 of granules of médium size 1,3 ^ to i,S \>- in diameter in the neighbourhood 

 of the nucleus, and between it and the free extremity of the cell, the tip 

 and sides containing only tiny granules which stain with difficulty. 



The protoplasm has often a condensed appearance but hardly stains 

 at ail. The vacuoles in which the zymogen granules lie appear collapsed in 

 many cases. The cell wall is unchanged in colour. 



