448 E -WAGE CARLIER 



Goblet Cells. 



Situated immediately below the cells just described and between thcm 

 and the oxyntic cells in the glands of the upper part of the stomach are a 

 few large mucin forming goblet cells, that are very conspicuous objects in 

 methyl blue eosine stained préparations owing to the peculiar peacock blue 

 colour of the material contained in their cups. This material would appear 

 never to be in the form of granules and refuses to stain with most dyes for 

 which mucigen exhibits affînities. With my méthylène blue patent B method 

 and with indulin they remain absolutely unstained indicating, that the 

 mucin contained in them is of a différent nature to that produced by the sur- 

 face and neck cells. They appear to behave to most dyes in a manner some- 

 what similar to that exhibited by the mucous cells lining the muco-salivary 

 glands of vertebrates, yet again they differ from them in their reaction to 

 some other dyes. 



The attached protoplasmic end of the cell is short and may be reduced 

 to a crescent at the base of the cup as seen in section, or it may be larger 

 and drawn out to a blunt point at the inner end. In this protoplasm lies the 

 nucleus which is of considérable size, triangular or flattened in section and 

 sometimes occupying such an amount of space that the protoplasm round 

 it is difficult to detect. In the resting nucleus chromatin is abundant and the 

 lanthanin granules numerous. Very small nucleoli are présent to the num- 

 ber of two or three, but in many cases only one is seen. 



The cup would appear to possess an open mouth like the chalice cells 

 described by Gilson in invertebrates (La Cellule), though in a few cases 

 an appearance suggesting the présence hère also of a perforated lid may be 

 seen. When fixed in corrosive sublimate the cups appear figured, i. e. they 

 présent a coarse irregular close meshed network and it is this meshwork that 

 stains, the material in its meshes remaining in ail cases, with the dyes used 

 by me, quite colourless. 



Thèse cells are especially abundant near the œsophageal end of the 

 stomach, but may be found throughout the organ, becoming rarer and rarer 

 as the pyloric end is reached; the}' are entirely absent from the pyloric glands 

 themselves. They appear to undergo division by mitosis, but karyokinetic 

 figures in them are very rare. 



During activity thèse cells appear at first to increase somewhat in size, 

 but soon again diminish, at the same time presenting a less brilliant 

 coloration and washed out appearance. The stainable network of the cups 



