450 B. ^WAGE CARLIER 



very rapidly as soon as the maximum of the secretory wave is passed; this 

 is indicated by the staining reaction which changes from feeble affinity for 

 the blue dye to an affinity for the acid eosine, whereby the protoplasm 

 stains somewhat reddish or reddish grey ; indicating probably that an 

 absorption is taking place from the blood of alkaline lymph into the pro- 

 toplasm. 



I think it would be more correctly stated if one said that repair goes 

 on during sécrétion, i. e. prozymogen is produced by the nuclei at the same 

 time that the zymogen already formed is being secreted by the cell in the 

 form of zymin. 



VVith regard to the nuclei, they increase slightly in size at the very com- 

 mencement of sécrétion, but soon become smaller and smaller and finally 

 show great wrinkling and puckering of the nuclear wall (Heidenhain ", 

 Hermann'% Schieferdecker-*, Mann", etc.) owing to diminution of their 

 contents. When repair sets in, which it does soon after it has begun in the 

 protoplasm, the nuclei rapidly lose their wrinkled appearance and swell up 

 to a considérable size, becoming much bigger than at the begining of sécré- 

 tion, to again diminish somewhat as the resting stage is reached. 



During nuclear activity the chromatin spreads itself out upon the inner 

 surface of the nuclear membrane and becomes diminished in amount, losing 

 some of its constituents which are again readily replacable during récupéra- 

 tion ; its becomes spread out and pale looking as if filled with fluid as obser- 

 ved by Bataillon"- (1891) and further it undoubtedly moves about during 

 the process. 



Of still more interest is I think the fact that when the cell is nearly 

 exhausted the affinity of the chromatin for the blue dye diminishes, while 

 its affinity for acid eosine increases to such an extent that the spread out 

 karyosomes tend to stain, rather red than blue. This can only be due to 

 altération occurring in the constituents of the karyosomes. This red stage 

 however does not last long, the blue reaction coming quickly back again 

 soon after repair has set in. It is interesting to note in this respect that the 

 chromatin of the head of the spermatozoon shows the same affinity for acid 

 dyes; though chemically rich in nucleic acid. Of no less interest in connec- 

 tion with this is the growth and extrusion of nucleoli during the period of 

 nuclear activity, which goes on for some time also during the process ol 

 repair, getting less and less as the nucleus is nearing the resting condition. 



I believe this extrusion of nucleoli to be a constant phenomenon of nu- 

 clear activity as I hâve observed it in many différent kinds of cells. Henné- 



