no. 3602 CRAYFISHES — HOBBS, HOLT, AND WALTON 25 



First form males of C. carolinus have been collected in the Mountain 

 Lake area in June, July, and August (elsewhere also in April and 

 September through December). A female with eggs was found in 

 June, and the young mentioned above in July. 



Cambarus longulus longulus Girard 



Figure 4 

 Cambarus longulus Girard, 1852, p. 90. 

 Cambarus longulus longulus Hay, 1899, p. 959 [by implication]. 



Diagnosis. — Margins of rostrum gently convergent from base to 

 apex, distinctly thickened and without marginal spines; areola never 

 more than six times longer than broad, usually about three and a 

 half, with crowded deep punctations; suborbital angle lacking. 

 Chela with subcylindrical fingers lacking distinct median longitudinal 

 ridges on upper surfaces; fingers gaping and usually provided basally 

 with conspicuous tuft of setae; inner margin of palm with single, 

 strongly depressed, row of tubercles (frequently scarcely rising above 

 contour of palm). 



Range. — In the Atlantic watershed from the James River in 

 Virginia to the Yadkin River in North Carolina in the low mountains 

 and upper piedmont provinces. Details of range and variation of 

 this and the other two subspecies of C. longulus are discussed by 

 James (1966). 



Specimens examined. — Approximately 450 specimens in 35 collec- 

 tions from the following stations: 6, 7, 9-13, 20-23, 25, 27, 29-35, 37, 

 88-93, 112, 123, and 124 in tributaries of the James River and North 

 Fork of the Roanoke River in Craig, Giles, and Montgomery Coun- 

 ties, Va., and from tributaries of Potts Creek, Monroe County, 

 W. Va. These stations lie at altitudes between 402 and 686 m. 



Associates. — Crayfish associates include C. acuminatus at stations 

 20, 27, 29-33, 35, 88-93, 112; C. b. bartonii at 6, 7, 9, 10, 12, 21-23, 

 25, 29, 32, 34, 35, 37, 89-93, 123, 124; and 0. juvenilis at 7. 



In the 23 localities in the James drainage, C. I. longulus was found 

 -m.th.Dn. ardis at stations 10-13, 20, 21, 23, 25, 27, 30-33, 35, 37, 123, 

 124; Dn. ileata at 6, 7, 9-12, 20-23, 25, 29, 31-35, 37, 123, 124; Dt. 

 suteri at 27, 30, 31, 33, 35, 124; and E. internotalus at 23, 30, 31, and 

 35. In the seven localities in the North Fork of the Roanoke River, 

 C. I. longulus was associated with Dt. jalcata at all stations (88-93, 

 112); Dn. truncata at 88, 90, 91, 93; and An. ancyla at 88, 89, 92, and 

 93. 



Branchiobdellid associates in the James drainage include A. koro- 

 naeus at stations 29, 30; B. illuminatus at 20, 22, 34; C. branchiophila 

 at 20, 29; C. heterognatha at 34, 123; C. holostoma at 7, 10, 11, 13, 

 20-23, 25, 37; C. philadelphica at 9, 10, 12, 20-22, 25, 27, 29, 32-35, 



