JOURNAL OF THE 



and the whole diluted to 100 cubic centimeters. This solution 

 should be prepared fresh every week. 



10 cubic centim<ters of the solution is brought to boil in a porce- 

 lain dish and urine added from a burette, until the last trace of 

 blue has disappeared from the solution. If the urine contains so 

 much sugar that less than three or four cubic centimeters precip- 

 itate all the copper, as is usually the case in diabetes mellitus, the 

 urine should be diluted with nine parts water, and this ^^ solution 

 used instead of the undiluted urine. 



It requires .005 gram sugar to reduce each cubic centimeter of 

 the Fehling solution. The following table indicates the number of 

 grams sugar per litre urine for each number of cubic centimeters 

 of the j\, urine solution used to reduce 10 cubic centimeters of Feh- 



To reduce from grams per litre to grains per fluid ounce multi- 

 ply by 1.8. 



While adding the urine the Fehling solution should be kept at 

 boiling point, but not allowed to boil. A gentle stirring facilitates 

 the settling of the red oxide of copper. 



To save time it is well to make a rough determination, first 

 adding one to two cubic centimeters at a time, and then to make 

 a second determination, adding at once nearly sufficient urine to 

 cause complete precipitation and then add very slowly 'till the 

 copper is accurately precipitated— a point which a little experience 

 will exactly determine. Two such determinations can be easily 

 made in fifteen minutes. 



If it is desirable to work wholly in the English system, the 

 following may be used : 



70 grains copper sulphate, 1^ fluid dram glycerine, 3 fluid ounces 



