ENTOMOPHTHOREAE OF THE UNITED STATES. 167 



The resting spores of this species occurred in several specimens simultaneously with 

 conidia, but were in every case too far advanced in their development to demonstrate 

 their process of formation, which can hardly vary greatly from that of the nearly allied 

 E. ajyiculata. 



Empusa Caroliniana nov. sp. 



PI. IG, figs. 91-105. 



Conidia ovoid, oblong or long elliptical, with rounded extremities, the base hardly sep- 

 arable from the apex; without large oil globules; measuring 10 X 2G,a - 15 X 45/jt, average 

 14 X 37//. Conidiojjhores simple, barely projecting beyond the body of the host between 

 the body segments and from the thorax; originating directly from rounded hyphal bod- 

 ies. Secondary conidia like the primary. Resting spores (azygospores) spherical, 

 hyaline, 37-55// in diameter, average 15,/ (?). Host attached to substratum by its legs. 



Hosts. Diptera: imagines of Tipula sp. 



Habitat. North Carolina. 



This species is decidedly different from any form known to me and is easily recognized 

 by its shapeless conidia which, although they are of the EntomopMhora rather than Em- 

 j)Hsa type, are borne on conidiophores which are rarely bi'anched within the body of the 

 host and are perfectly simple at their extremities ; the hyphae swelling directly to a ba- 

 sidium upon emerging. Specimens producing the fungus with the greatest luxuriance may 

 therefore show little indication of its presence, and in dried material it is almost wholly 

 invisible, the body segments closing over the basidia in shrinking. A single specimen 

 only was taken at CuUowhee, while at Cranberry I ])rocured a considerable amount of 

 excellent material. The affected insects (a brown Tipula with mottled brown wings) 

 were found in a small group of hemlocks among deciduous woods and occurred invaria- 

 bly hanging from the lower dead twigs, about which the extremities of their long legs 

 were knotted in such a manner that it was impossible to disengage them. Several spec- 

 imens contained only hyphal bodies and I was thus enabled to see the greatest possible 

 development of conidiophores, by i^lacing the Tipidae in a moist atmosphere. Other 

 specimens produced both conidia and resting spores, although I was unable to make out 

 the origin of the latter. As far as could be judged from the examination of material 

 in which the process was already completed, it consisted in a budding from rounded hy- 

 phal bodies, but no satisfactory observation was possible in any case. The terminal and 

 interstitial production of hyphal bodies (fig. 92) through the successive contraction of 

 the contents of branching hyphae was frequently observed ; but in other cases rounded 

 hyphal bodies occurred, associated with resting spores, which Avere much larger than 

 those represented in the figure and may have had a different origin. 



Empusa (Triplosporium) Fresenii Nowakowski. 

 PI. IG, figs. IOG-140, 



Empusa Fresenii, ISTowakowski I. c. B, p. 171, PL xii, figs. 115-125. Schroeter I. c, 

 p. 222. 



