NORTH AMERICAN ANTHURUS. 501 



with the aid of an Abbe camera lucida, and shows accurately the differences in form between 

 the branched tramal hypha and the constricted basidium. But there are also differences 

 in nature between the two, which are revealed by the action of stains. The tramal 

 hyphae and their short branches as far as the first cell of the basidia stain but very 

 slightly with carmine or eosin, while the 4- and 5-celled basidium stains intensely with 

 these substances and its parts all stain alike, the terminal cell not differing in nature 

 from the three or four below. 



This character of the basidia seems to be unique — at least so far as my reading 

 and observation go. Still it may have been overlooked in other cases. It is readily 

 demonstrated by crushing under a cover-glass sections that have been treated with 

 potassium hydrate and then staining them with aqueous solution of eosin. 



No cystidia were to be seen. In my Fig. 11, I have omitted drawing the spores 

 on one basidium, but that was for clearness in the figure. The sterigmata are very 

 short, and the spores are borne in a close cluster of from 5 to 8 at the obtuse end of 

 the basidium. The spores are olive-green, simple, ellipsoidal, 3-4 X 1 1-2 jx. Throughout 

 the gleba they all seem to be in about the same stage of development and all ripen 

 together probably. No attempt has been made to germinate them. 



Clamp Connections. 



A form of clamp connection may be seen in the earlier stages of the egg. It 

 was observed very frequently in the medullary tissue of the axis of the plant and in 

 the gelatinous tissue of the periclium and more rarely in the cortical layer. In the 

 latter case hyphae having such connections showed fewer anastomoses with the other 

 hyphae and were more regular in form than is the case usually with the cortical hyphae. 

 In this form of clamp connection one or both of the adjacent cell ends are very 

 considerably swollen. Fig. 13 a shows one of the cases in the cortical layer and Fig. 

 13 b, two in the gelatinous layer of the periclium. 



Summary of Development. 



1. All of the tissues of the egg arise from internal differentiation of the medullary 

 and cortical tissues of the mycelial strand. In such differentiation 



(a) The medullary portion gives rise to the column of gelatinous tissue in the 

 main cavity of the stipe, to the more persistent forms of this tissue which constitute the 

 diaphragm and the dome, to the entire mass of the gleba,. and to the gelatinous and 

 inner layers of the peridium; while 



(6) The cortical layer gives rise to the outer wall of the peridium, to the cortical 

 plates (or radial walls), and to the cortical sheath of loose tissue outside of the stipe. 



