72 



BULLETIN" 120, UISTITED STATES NATIONAL MUSEUM. 



from Concepcion, Chile, no date given ; T. Barbour, collector. Two 

 other individuals wore opened; one contained numerous Zelleriella 

 danvinii; the other contained no Opalinids. 



Measurements of an individual of the prevalent sort. — Length of 

 body 0.119 mm.; width of body 0.03 mm.; thickness of body 0.0275 

 mm. ; length of nucleus 0.022 mm. ; width of nucleus 0.008 mm. ; 

 diameter of endospherule 0.00155 mm. ; cilia line interval, anterior 



0.00165 mm.; posterior 

 0.00305 mm. Massive chro- 

 mosomes six in number. 



This is a small species. 

 Most of the individuals in 

 my infection are of the sort 

 shown in figure 42, c. The 

 body is alwaj^s sharply 

 pointed behind, but there 

 is no naked spinelike proc- 

 ess. The nuclei are spindle- 

 shaped, and in all but a few 

 individuals are seen to be 

 in an anaphase stage, show- 

 ing clearly six massive 

 chromosomes. Granular 

 chromosomes can also be 

 seen, but the preparations 

 are not sufficiently clear to 

 allow counting them. A 

 few individuals are found, 

 with oval nuclei (fig. 42, a) 

 in which the chromosomes 

 seem to be fragmented, 

 probably passing from a 

 reticulate stage into active 

 mitosis. A very few others are found with nuclei as shown in figure 

 42, h. Portions of the excretory vacuole are often seen, especially 

 around the nuclei. 



a 



Fig. 43. — Peotoopalina longinucleata : a. An 



ORDINARY INDIVIDDAL, X 460 DIAMETERS ; b, ITS 

 ANTERIOR NUCLEUS, ONLY THE ANTERIOR END BE- 

 ING CLEARLY SEEN OWING TO THE OVERLAPPING OF 

 THE TWO NUCLEI, X 1,000 DIAMETERS ; C, A 

 DAUGHTER CELL, X 1,000 DIAMETERS. 



PROTOOPALINA LONGINUCLEATA, new species (flg. 43, above). 



Type. — United States National Musuem Cat. No. 16449. 



Host. — Telmatohius jelskii (Peters), one infection in United 

 States National Museum specimen No. 33864, 50 mm. long, from 

 Guamote, Ecuador, 10,000 feet elevation, October 10, 1903. Five 

 individuals from Ecuador and Peru were opened. One contained 

 P. longinucleata and four contained Zelleriella telmatohii. 



