THE OPALINIIi CILIATE INFUSOETAX.S. 



123 



ZELLERIELLA INTERMEDIA, new species. 



'J' j/pe.— United States National Museum Cat. No. 16486. 



Host.—Bufo intermedins Guenther, two abundant infections, from 

 United States National Museum specimens No. 26161 and 26162, 22 

 mm. lon<r (the type infection), both from Guanajuato, Mexico, A. 

 Duges, collector. 



Measurements of an average individudh — Length of body 0.0937 

 mm. ; width of bodj' 0.05 mm. ; thickness of body 0.016 mm. ; diameter 

 of resting nucleus 0.0109 mm. ; length of endospherule 0.0015 mm. ; 

 width of endospherule 0.001 mm. ; cilia line interval, anterior 0.0017 

 mm., middle 0.00285 mm., posterior 0.00225 mm. Four macrochromo- 

 somes. 



This is a narrow, wedge-shaped species. One little peculiarity is 

 observed, perhaps correlated Avith its form, namely, that the primary 



Fl J. 89. ZELLERIELLA INTERMEDIA, X 460 DIAMETERS: fl, SHOWS SOME OF THE LI.NB8 



OF INSEETION OF THE CILIA ; C IS A VERTICAL, OBLIQUE, OPTICAL SECTION. 



(major) lines of cilia are farther apart over the middle of the body 

 than they are behind. The nuclei are nearly spherical, but not quite 

 so. They are distinct from each other, not being connected by a 

 thread. Each shows four distinct chromatin masses which seem to be 

 macrochromosomes. These are arranged as in a very early metaphase, 

 with the chromos&mes distinct but irregularly placed. 



It seems appropriate to name this species intermedia^ both because 

 its host is Bufo intermedins and because in the size of its nuclei it is 

 intermediate between Z. hufoxena and Z. mlcrocarya, on the one 

 hand, and some other species soon to be described. 



ZELLERIELLA INTERMEDIA CUNEATA, new subspecies. 



7y;?e.— United States National Museum Cat. No. 16487. 

 Host. — Bufo vallicex^s Wiegmann, two abundant infections, from 

 Brownsville, Texas, in United States National Museum specimens 



